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dc.contributor.authorXu, Z
dc.contributor.authorZang, X
dc.contributor.authorZheng, Q
dc.contributor.authorLi, Y
dc.contributor.authorField, N
dc.contributor.authorFiserova, J
dc.contributor.authorHua, B
dc.contributor.authorKriechbaumer, V
dc.contributor.authorDeeks, MJ
dc.contributor.authorHussey, PJ
dc.contributor.authorWang, P
dc.date.accessioned2025-01-16T13:20:06Z
dc.date.issued2025-02-06
dc.date.updated2025-01-16T12:43:06Z
dc.description.abstractPlant cytokinesis requires coordination between the actin cytoskeleton, microtubules and membranes to guide division plane formation and cell plate expansion; how these regulatory factors are coordinated remains unknown. The actin cytoskeleton assembly is controlled by several actin nucleation factors, such as the SCAR/WAVE complex, which regulates actin nucleation and branching through the activation of the ARP2/3 complex. The activity of these actin regulatory proteins is likely influenced by interactions with specific membranes; however, the molecular basis and the biological relevance of SCAR-membrane interactions are also unclear. In this study, we demonstrate that the ER-PM tethering protein VAP27-1 directly interacts with SCAR2 at the ER membrane, and that they co-localise to guide cell plate orientation during cell division. In the root meristem, both VAP27-1 and SCAR2 exhibit polarised localisation at the cell plates, where the interaction between ER and PM is abundant. VAP27-1 recruits SCAR2 to the cell division plane, where there is a high concentration of actin filaments. In the vap27-1346 mutant, the densities of cortical ER, SCAR2 and consequently actin filaments are significantly reduced at the cell division plane, affecting cell plate orientation, cell division and root development. A similar phenomenon is also observed in the scar1234 mutant, suggesting that VAP27 and SCAR proteins regulate cell division through a similar pathway. In conclusion, our data reveal a plant-specific function of VAP27-regulated ER-PM interaction and advance our understanding of plant ER-PM contact site and its role in cell division.en_GB
dc.description.sponsorshipNational Natural Science Foundation of China (NSFC)en_GB
dc.description.sponsorshipFundamental Research Funds for the Central Universitiesen_GB
dc.description.sponsorshipYoung Scientist Fostering Funds for the National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Cropsen_GB
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BBSRC)en_GB
dc.identifier.citationVol. 122 (6), article e2416927122en_GB
dc.identifier.doi10.1073/pnas.2416927122
dc.identifier.grantnumber92254307en_GB
dc.identifier.grantnumber32261160371en_GB
dc.identifier.grantnumber2662023PY011en_GB
dc.identifier.grantnumberHorti-550 PY-2023-001en_GB
dc.identifier.grantnumberBBC516601/1en_GB
dc.identifier.urihttp://hdl.handle.net/10871/139656
dc.identifierORCID: 0000-0001-5487-5732 (Deeks, Michael)
dc.language.isoenen_GB
dc.publisherNational Academy of Sciencesen_GB
dc.rights© 2025 The author(s). For the purpose of open access, the author has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising from this submission.
dc.subjectER-PM contact sitesen_GB
dc.subjectActin cytoskeletonen_GB
dc.subjectEndoplasmic Reticulumen_GB
dc.subjectSCAR/WAVE complexen_GB
dc.subjectCytokinesisen_GB
dc.subjectCell plateen_GB
dc.titleThe ER-PM interaction is essential for cytokinesis and recruits the actin cytoskeleton through the SCAR/WAVE complexen_GB
dc.typeArticleen_GB
dc.date.available2025-01-16T13:20:06Z
dc.identifier.issn0027-8424
dc.descriptionThis is the author accepted manuscript. The final version is available from the National Academy of Sciences via the DOI in this recorden_GB
dc.descriptionData share plans: All study data are included in the article and/or supporting information.en_GB
dc.identifier.eissn1091-6490
dc.identifier.journalProceedings of the National Academy of Sciences (PNAS)en_GB
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_GB
dcterms.dateAccepted2024-12-27
dcterms.dateSubmitted2024-08-21
rioxxterms.versionAMen_GB
rioxxterms.licenseref.startdate2024-12-27
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2025-01-16T13:11:01Z
refterms.versionFCDAM
refterms.dateFOA2025-02-11T16:03:33Z
refterms.panelAen_GB
exeter.rights-retention-statementNo


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© 2025 The author(s). For the purpose of open access, the author has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising from this submission.
Except where otherwise noted, this item's licence is described as © 2025 The author(s). For the purpose of open access, the author has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising from this submission.