The ER-PM interaction is essential for cytokinesis and recruits the actin cytoskeleton through the SCAR/WAVE complex
dc.contributor.author | Xu, Z | |
dc.contributor.author | Zang, X | |
dc.contributor.author | Zheng, Q | |
dc.contributor.author | Li, Y | |
dc.contributor.author | Field, N | |
dc.contributor.author | Fiserova, J | |
dc.contributor.author | Hua, B | |
dc.contributor.author | Kriechbaumer, V | |
dc.contributor.author | Deeks, MJ | |
dc.contributor.author | Hussey, PJ | |
dc.contributor.author | Wang, P | |
dc.date.accessioned | 2025-01-16T13:20:06Z | |
dc.date.issued | 2025-02-06 | |
dc.date.updated | 2025-01-16T12:43:06Z | |
dc.description.abstract | Plant cytokinesis requires coordination between the actin cytoskeleton, microtubules and membranes to guide division plane formation and cell plate expansion; how these regulatory factors are coordinated remains unknown. The actin cytoskeleton assembly is controlled by several actin nucleation factors, such as the SCAR/WAVE complex, which regulates actin nucleation and branching through the activation of the ARP2/3 complex. The activity of these actin regulatory proteins is likely influenced by interactions with specific membranes; however, the molecular basis and the biological relevance of SCAR-membrane interactions are also unclear. In this study, we demonstrate that the ER-PM tethering protein VAP27-1 directly interacts with SCAR2 at the ER membrane, and that they co-localise to guide cell plate orientation during cell division. In the root meristem, both VAP27-1 and SCAR2 exhibit polarised localisation at the cell plates, where the interaction between ER and PM is abundant. VAP27-1 recruits SCAR2 to the cell division plane, where there is a high concentration of actin filaments. In the vap27-1346 mutant, the densities of cortical ER, SCAR2 and consequently actin filaments are significantly reduced at the cell division plane, affecting cell plate orientation, cell division and root development. A similar phenomenon is also observed in the scar1234 mutant, suggesting that VAP27 and SCAR proteins regulate cell division through a similar pathway. In conclusion, our data reveal a plant-specific function of VAP27-regulated ER-PM interaction and advance our understanding of plant ER-PM contact site and its role in cell division. | en_GB |
dc.description.sponsorship | National Natural Science Foundation of China (NSFC) | en_GB |
dc.description.sponsorship | Fundamental Research Funds for the Central Universities | en_GB |
dc.description.sponsorship | Young Scientist Fostering Funds for the National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.identifier.citation | Vol. 122 (6), article e2416927122 | en_GB |
dc.identifier.doi | 10.1073/pnas.2416927122 | |
dc.identifier.grantnumber | 92254307 | en_GB |
dc.identifier.grantnumber | 32261160371 | en_GB |
dc.identifier.grantnumber | 2662023PY011 | en_GB |
dc.identifier.grantnumber | Horti-550 PY-2023-001 | en_GB |
dc.identifier.grantnumber | BBC516601/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/139656 | |
dc.identifier | ORCID: 0000-0001-5487-5732 (Deeks, Michael) | |
dc.language.iso | en | en_GB |
dc.publisher | National Academy of Sciences | en_GB |
dc.rights | © 2025 The author(s). For the purpose of open access, the author has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising from this submission. | |
dc.subject | ER-PM contact sites | en_GB |
dc.subject | Actin cytoskeleton | en_GB |
dc.subject | Endoplasmic Reticulum | en_GB |
dc.subject | SCAR/WAVE complex | en_GB |
dc.subject | Cytokinesis | en_GB |
dc.subject | Cell plate | en_GB |
dc.title | The ER-PM interaction is essential for cytokinesis and recruits the actin cytoskeleton through the SCAR/WAVE complex | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2025-01-16T13:20:06Z | |
dc.identifier.issn | 0027-8424 | |
dc.description | This is the author accepted manuscript. The final version is available from the National Academy of Sciences via the DOI in this record | en_GB |
dc.description | Data share plans: All study data are included in the article and/or supporting information. | en_GB |
dc.identifier.eissn | 1091-6490 | |
dc.identifier.journal | Proceedings of the National Academy of Sciences (PNAS) | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2024-12-27 | |
dcterms.dateSubmitted | 2024-08-21 | |
rioxxterms.version | AM | en_GB |
rioxxterms.licenseref.startdate | 2024-12-27 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2025-01-16T13:11:01Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2025-02-11T16:03:33Z | |
refterms.panel | A | en_GB |
exeter.rights-retention-statement | No |
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Except where otherwise noted, this item's licence is described as © 2025 The author(s). For the purpose of open access, the author has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising from this submission.