dc.contributor.author | Skrzypiec, AE | |
dc.contributor.author | Shah, RS | |
dc.contributor.author | Schiavon, E | |
dc.contributor.author | Baker, E | |
dc.contributor.author | Skene, N | |
dc.contributor.author | Pawlak, R | |
dc.contributor.author | Mucha, M | |
dc.date.accessioned | 2013-12-03T14:11:07Z | |
dc.date.issued | 2013-04 | |
dc.description.abstract | Behavioural adaptation to psychological stress is dependent on neuronal plasticity and dysfunction at this cellular level may underlie the pathogenesis of affective disorders such as depression and post-traumatic stress disorder. Taking advantage of genome-wide microarray assay, we performed detailed studies of stress-affected transcripts in the amygdala - an area which forms part of the innate fear circuit in mammals. Having previously demonstrated the role of lipocalin-2 (Lcn-2) in promoting stress-induced changes in dendritic spine morphology/function and neuronal excitability in the mouse hippocampus, we show here that the Lcn-2 gene is one of the most highly upregulated transcripts detected by microarray analysis in the amygdala after acute restraint-induced psychological stress. This is associated with increased Lcn-2 protein synthesis, which is found on immunohistochemistry to be predominantly localised to neurons. Stress-naïve Lcn-2(-/-) mice show a higher spine density in the basolateral amygdala and a 2-fold higher rate of neuronal firing rate compared to wild-type mice. Unlike their wild-type counterparts, Lcn-2(-/-) mice did not show an increase in dendritic spine density in response to stress but did show a distinct pattern of spine morphology. Thus, amygdala-specific neuronal responses to Lcn-2 may represent a mechanism for behavioural adaptation to psychological stress. | en_GB |
dc.description.sponsorship | Marie Curie Excellence Grant from the European Commission. | |
dc.description.sponsorship | Medical Research Council Project Grant | |
dc.description.sponsorship | COST Action ECMNet | |
dc.identifier.citation | PloS One, 2013, Vol. 8, Issue 4 | en_GB |
dc.identifier.doi | 10.1371/journal.pone.0061046 | |
dc.identifier.grantnumber | MEXT-CT-2006-042265 | |
dc.identifier.grantnumber | G0500231/73852 | |
dc.identifier.grantnumber | BM1001 | |
dc.identifier.uri | http://hdl.handle.net/10871/14132 | |
dc.language.iso | en | en_GB |
dc.publisher | Public Library of Science | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/23593384 | en_GB |
dc.relation.url | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0061046 | en_GB |
dc.rights | © 2013 Skrzypiec et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
dc.subject | Action Potentials | en_GB |
dc.subject | Alternative Splicing | en_GB |
dc.subject | Amygdala | en_GB |
dc.subject | Animals | en_GB |
dc.subject | Cluster Analysis | en_GB |
dc.subject | Dendritic Spines | en_GB |
dc.subject | Gene Expression Profiling | en_GB |
dc.subject | Gene Expression Regulation | en_GB |
dc.subject | Lipocalins | en_GB |
dc.subject | Male | en_GB |
dc.subject | Mice | en_GB |
dc.subject | Mice, Knockout | en_GB |
dc.subject | Neurons | en_GB |
dc.subject | Stress, Psychological | en_GB |
dc.subject | Transcription, Genetic | en_GB |
dc.title | Stress-induced lipocalin-2 controls dendritic spine formation and neuronal activity in the amygdala. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2013-12-03T14:11:07Z | |
exeter.place-of-publication | United States | |
dc.description | This is a freely-available open access publication. Please cite the published version which is available via the DOI link in this record. | en_GB |
dc.identifier.journal | PLoS One | en_GB |