Identification of a key residue for oligomerisation and pore-formation of Clostridium perfringens NetB.
Fernandes da Costa, Sérgio P.
Savva, Christos G.
Naylor, Claire E.
Moss, David S.
Basak, Ajit K.
Titball, Richard W.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-directed mutagenesis was used to identify amino acids that play a role in NetB oligomerisation and pore-formation. NetB K41H showed significantly reduced toxicity towards LMH cells and human red blood cells relative to wild type toxin. NetB K41H was unable to oligomerise and form pores in liposomes. These findings suggest that NetB K41H could be developed as a genetic toxoid vaccine to protect against necrotic enteritis.
European Union Marie Curie Network
This is an open access article that is freely available in ORE or from the publisher's web site. Please cite the published version.
© 2014 by the authors; licensee MDPI, Basel, Switzerland
Open Access Toxinology Journal
Vol. 6, Issue 3, pp. 1049 - 1061
PubMed Central ID
Place of publication