dc.contributor.author | Dagdas, Yasin Fatih | |
dc.contributor.author | Yoshino, K | |
dc.contributor.author | Dagdas, Gulay | |
dc.contributor.author | Ryder, LS | |
dc.contributor.author | Bielska, Ewa | |
dc.contributor.author | Steinberg, Gero | |
dc.contributor.author | Talbot, Nicholas J. | |
dc.date.accessioned | 2014-11-19T11:14:37Z | |
dc.date.issued | 2012-06-22 | |
dc.description.abstract | To cause rice blast disease, the fungus Magnaporthe oryzae develops a pressurized dome-shaped cell called an appressorium, which physically ruptures the leaf cuticle to gain entry to plant tissue. Here, we report that a toroidal F-actin network assembles in the appressorium by means of four septin guanosine triphosphatases, which polymerize into a dynamic, hetero-oligomeric ring. Septins scaffold F-actin, via the ezrin-radixin-moesin protein Tea1, and phosphatidylinositide interactions at the appressorium plasma membrane. The septin ring assembles in a Cdc42- and Chm1-dependent manner and forms a diffusion barrier to localize the inverse-bin-amphiphysin-RVS-domain protein Rvs167 and the Wiskott-Aldrich syndrome protein Las17 at the point of penetration. Septins thereby provide the cortical rigidity and membrane curvature necessary for protrusion of a rigid penetration peg to breach the leaf surface. | |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | |
dc.description.sponsorship | ERC | |
dc.identifier.citation | Vol. 336, Issue 6088, pp. 1590 - 1595 | |
dc.identifier.doi | 10.1126/science.1222934 | |
dc.identifier.uri | http://hdl.handle.net/10871/15897 | |
dc.language.iso | en | |
dc.publisher | American Association for the Advancement of Science (AAAS) | |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/22723425 | |
dc.subject | Actin Cytoskeleton | |
dc.subject | Actins | |
dc.subject | Cell Membrane | |
dc.subject | Diffusion | |
dc.subject | Fungal Proteins | |
dc.subject | Magnaporthe | |
dc.subject | Microfilament Proteins | |
dc.subject | Mutation | |
dc.subject | Oryza sativa | |
dc.subject | Phosphatidylinositols | |
dc.subject | Plant Diseases | |
dc.subject | Plant Leaves | |
dc.subject | Protein Interaction Domains and Motifs | |
dc.subject | Recombinant Fusion Proteins | |
dc.subject | Septins | |
dc.subject | cdc42 GTP-Binding Protein | |
dc.title | Septin-mediated plant cell invasion by the rice blast fungus, Magnaporthe oryzae | |
dc.type | Article | |
dc.date.available | 2014-11-19T11:14:37Z | |
exeter.place-of-publication | United States | |
dc.description | This is the author accepted manuscript. The final version is available from the American Association for the Advancement of Science via the DOI in this record | |
dc.identifier.journal | Science | |
refterms.dateFOA | 2024-08-07T18:00:56Z | |