dc.contributor.author | Van Oers, JMM | |
dc.contributor.author | Edwards, Y | |
dc.contributor.author | Chahwan, R | |
dc.contributor.author | Zhang, W | |
dc.contributor.author | Smith, C | |
dc.contributor.author | Pechuan, X | |
dc.contributor.author | Schaetzlein, S | |
dc.contributor.author | Jin, B | |
dc.contributor.author | Wang, Yuxun | |
dc.contributor.author | Bergman, A | |
dc.contributor.author | Scharff, MD | |
dc.contributor.author | Edelmann, W | |
dc.date.accessioned | 2015-03-09T11:30:19Z | |
dc.date.issued | 2014-07-24 | |
dc.description.abstract | Loss of the DNA mismatch repair protein MSH3 leads to the development of a variety of tumors in mice without significantly affecting survival rates, suggesting a modulating role for the MutSβ (MSH2-MSH3) complex in late onset tumorigenesis. To better study the role of MSH3 in tumor progression, we crossed Msh3−/− mice onto a tumor predisposing p53-deficient background. Survival of Msh3/p53 mice was not reduced compared to single p53 mutant mice; however, the tumor spectrum changed significantly from lymphoma to sarcoma, indicating MSH3 as a potent modulator of p53-driven tumorigenesis. Interestingly, Msh3−/− mouse embryonic fibroblasts displayed increased chromatid breaks and persistence of γH2AX foci following ionizing radiation, indicating a defect in DNA double strand break repair. Msh3/p53 tumors showed increased loss of heterozygosity, elevated genome-wide copy number variation, and a moderate microsatellite instability phenotype compared to Msh2/p53 tumors, revealing that MSH2-MSH3 suppresses tumorigenesis by maintaining chromosomal stability. Our results show that the MSH2-MSH3 complex is important for the suppression of late onset tumors due to its role in DNA double strand break repair as well as in DNA mismatch repair. Furthermore, they demonstrate that MSH2-MSH3 suppresses chromosomal instability and modulates the tumor spectrum in p53-deficient tumorigenesis, and possibly plays a role in other chromosomally unstable tumors as well. | en_GB |
dc.description.sponsorship | National Institutes of Health | en_GB |
dc.identifier.citation | Vol. 33, Issue 30, pp. 3939 - 3946 | en_GB |
dc.identifier.doi | 10.1038/onc.2013.365 | |
dc.identifier.grantnumber | CA76329 | en_GB |
dc.identifier.grantnumber | CA93484 | en_GB |
dc.identifier.grantnumber | CA72649 | en_GB |
dc.identifier.grantnumber | CA102705 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/16470 | |
dc.language.iso | en | en_GB |
dc.publisher | Nature Publishing Group | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3961563/ | en_GB |
dc.relation.url | http://www.nature.com/onc/journal/v33/n30/full/onc2013365a.html | en_GB |
dc.subject | DNA mismatch repair | en_GB |
dc.subject | MSH2-MSH3 | en_GB |
dc.subject | DNA double strand break repair | en_GB |
dc.subject | chromosomal instability | en_GB |
dc.subject | p53 | en_GB |
dc.subject | sarcomagenesis | en_GB |
dc.title | The MutSβ complex is a modulator of p53-driven tumorigenesis through its functions in both DNA double-strand break repair and mismatch repair | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2015-03-09T11:30:19Z | |
dc.identifier.issn | 0950-9232 | |
dc.description | This a post-print, author-produced version of an article accepted for publication in Oncogene. Copyright © 2014 Nature Publishing Group. The definitive version is available at http://www.nature.com/onc/journal/v33/n30/full/onc2013365a.html | en_GB |
dc.identifier.journal | Oncogene | en_GB |