dc.contributor.author | Ahmed, U | |
dc.contributor.author | Anwar, A | |
dc.contributor.author | Savage, RS | |
dc.contributor.author | Costa, Matthew L. | |
dc.contributor.author | Mackay, N | |
dc.contributor.author | Filer, A | |
dc.contributor.author | Raza, K | |
dc.contributor.author | Watts, RA | |
dc.contributor.author | Winyard, Paul G. | |
dc.contributor.author | Tarr, J | |
dc.contributor.author | Haigh, Richard | |
dc.contributor.author | Thornalley, PJ | |
dc.contributor.author | Rabbani, N | |
dc.date.accessioned | 2015-04-13T09:23:07Z | |
dc.date.issued | 2015-03-19 | |
dc.description.abstract | There is currently no biochemical test for detection of early-stage osteoarthritis (eOA). Tests for early-stage rheumatoid arthritis (eRA) such as rheumatoid factor (RF) and anti-cyclic citrullinated peptide (CCP) antibodies require refinement to improve clinical utility. We developed robust mass spectrometric methods to quantify citrullinated protein (CP) and free hydroxyproline in body fluids. We detected CP in the plasma of healthy subjects and surprisingly found that CP was increased in both patients with eOA and eRA whereas anti-CCP antibodies were predominantly present in eRA. A 4-class diagnostic algorithm combining plasma/serum CP, anti-CCP antibody and hydroxyproline applied to a cohort gave specific and sensitive detection and discrimination of eOA, eRA, other non-RA inflammatory joint diseases and good skeletal health. This provides a first-in-class plasma/serum-based biochemical assay for diagnosis and type discrimination of early-stage arthritis to facilitate improved treatment and patient outcomes, exploiting citrullinated protein and related differential autoimmunity. | en_GB |
dc.identifier.citation | Vol. 5 article 9259 | en_GB |
dc.identifier.doi | 10.1038/srep09259 | |
dc.identifier.uri | http://hdl.handle.net/10871/16746 | |
dc.language.iso | en | en_GB |
dc.publisher | Nature Publishing Group | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/25788417 | en_GB |
dc.rights | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en_GB |
dc.title | Biomarkers of early stage osteoarthritis, rheumatoid arthritis and musculoskeletal health | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2015-04-13T09:23:07Z | |
dc.identifier.issn | 2045-2322 | |
exeter.place-of-publication | England | |
dc.description | This is a freely-available open access publication. Please cite the published version which is available via the DOI link in this record. | en_GB |
dc.identifier.journal | Scientific Reports | en_GB |