Differential immuno-kinetic assays of allergen-specific binding for peanut allergy serum analysis.
Analytical and Bioanalytical Chemistry
Springer Verlag (Germany)
The final publication is available at Springer via http://dx.doi.org/ 10.1007/s00216-012-6390-z
A label-free nanoparticle array platform has been used to detect total peanut allergen-specific binding from whole serum of patients suffering from peanut allergy. The serum from 10 patients was screened against a four-allergen panel of cat and dog dander, dust mite and peanut allergen protein Ara h1. The IgE and IgG contributions to the total specific-binding protein load to Ara h1 were identified using two secondary IgG- and IgE-specific antibodies and were found to contribute less than 50 % of the total specific protein load. The total mass of IgE, IgE and the unresolved specific-binding protein ΔsBP for Ara h1 provides a new serum profile for high-RAST-grade patients 5 and 6 with the IgG/IgE ratio of 4 ± 2 and ΔsBP/IgE ratio of 17 ± 11, neither of which is protective for the small patient cohort.
Research Support, Non-U.S. Gov't
Copyright © Springer-Verlag 2012
Vol. 404, Iss. 8, pp. 2241 - 2247
Place of publication