dc.contributor.author | Boots, M | |
dc.contributor.author | Baggart, S | |
dc.contributor.author | Tidbury, H | |
dc.contributor.author | Bridgett, S | |
dc.contributor.author | Garbutt, J | |
dc.contributor.author | Kaur, G | |
dc.date.accessioned | 2015-05-08T13:40:47Z | |
dc.date.issued | 2015-04-17 | |
dc.description.abstract | Background
Little is known about invertebrate responses to DNA viruses. Here, we infect a commercially important pest moth species Plodia interpunctella with its naturally infecting DNA virus. We sequenced, assembled and annotated the complete transcriptome of the moth, and a partial transcriptome of the virus. We then tested for differential gene expression between moths that were exposed to the virus and controls.
Results
We found 51 genes that were differentially expressed in moths exposed to a DNA baculovirus compared to controls. Gene set enrichment analysis revealed that cuticle proteins were significantly overrepresented in this group of genes. Interestingly, 6 of the 7 differentially expressed cuticle proteins were downregulated, suggesting that baculoviruses are able to manipulate its host’s response. In fact, an additional 29 of the 51 genes were also downregulated in exposed compared with control animals, including a gram-negative binding protein. In contrast, genes involved in transposable element movement were upregulated after infection.
Conclusions
We present the first experiment to measure genome-wide gene expression in an insect after infection with a natural DNA virus. Our results indicate that cuticle proteins might be key genes underpinning the response to DNA viruses. Furthermore, the large proportion of genes that were downregulated after viral exposure suggests that this virus is actively manipulating the insect immune response. Finally, it appears that transposable element activity might increase during viral invasion. Combined, these results provide much needed host candidate genes that respond to DNA viral invaders. | en_GB |
dc.description.sponsorship | NERC Biomolecular Analysis Facility (NBAF) | en_GB |
dc.identifier.citation | Vol. 16 (1), article 310 | en_GB |
dc.identifier.doi | 10.1186/s12864-015-1499-z | |
dc.identifier.uri | http://hdl.handle.net/10871/17148 | |
dc.language.iso | en | en_GB |
dc.publisher | BioMed Central | en_GB |
dc.relation.url | http://www.biomedcentral.com/1471-2164/16/310 | en_GB |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | en_GB |
dc.subject | Differential gene expression | en_GB |
dc.subject | DNA virus | en_GB |
dc.subject | de novo transcriptome assembly | en_GB |
dc.subject | host-pathogen interaction | en_GB |
dc.subject | RNA-Seq | en_GB |
dc.title | Novel insights into the insect transcriptome response to a natural DNA virus | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2015-05-08T13:40:47Z | |
dc.identifier.issn | 1471-2164 | |
dc.description | Article | en_GB |
dc.description | Copyright © 2015 McTaggart et al.; licensee BioMed Central. | en_GB |
dc.identifier.journal | BMC Genomics | en_GB |