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dc.contributor.authorStudholme, DJ
dc.contributor.authorBentley, SD
dc.contributor.authorKormanec, J
dc.date.accessioned2015-06-12T15:14:36Z
dc.date.issued2004-04-08
dc.description.abstractBACKGROUND: Streptomyces coelicolor is a bacterium with a vast repertoire of metabolic functions and complex systems of cellular development. Its genome sequence is rich in genes that encode regulatory proteins to control these processes in response to its changing environment. We wished to apply a recently published bioinformatic method for identifying novel regulatory sequence signals to gain new insights into regulation in S. coelicolor. RESULTS: The method involved production of position-specific weight matrices from alignments of over-represented words of DNA sequence. We generated 2497 weight matrices, each representing a candidate regulatory DNA sequence motif. We scanned the genome sequence of S. coelicolor against each of these matrices. A DNA sequence motif represented by one of the matrices was found preferentially in non-coding sequences immediately upstream of genes involved in polysaccharide degradation, including several that encode chitinases. This motif (TGGTCTAGACCA) was also found upstream of genes encoding components of the phosphoenolpyruvate phosphotransfer system (PTS). We hypothesise that this DNA sequence motif represents a regulatory element that is responsive to availability of carbon-sources. Other motifs of potential biological significance were found upstream of genes implicated in secondary metabolism (TTAGGTtAGgCTaACCTAA), sigma factors (TGACN19TGAC), DNA replication and repair (ttgtCAGTGN13TGGA), nucleotide conversions (CTACgcNCGTAG), and ArsR (TCAGN12TCAG). A motif found upstream of genes involved in chromosome replication (TGTCagtgcN7Tagg) was similar to a previously described motif found in UV-responsive promoters. CONCLUSIONS: We successfully applied a recently published in silico method to identify conserved sequence motifs in S. coelicolor that may be biologically significant as regulatory elements. Our data are broadly consistent with and further extend data from previously published studies. We invite experimental testing of our hypotheses in vitro and in vivo.en_GB
dc.description.sponsorshipMRCen_GB
dc.identifier.citationVol. 4, pp. 14 -en_GB
dc.identifier.doi10.1186/1471-2180-4-14
dc.identifier.other1471-2180-4-14
dc.identifier.urihttp://hdl.handle.net/10871/17523
dc.language.isoenen_GB
dc.publisherBioMed Centralen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/15072583en_GB
dc.relation.urlhttp://www.biomedcentral.com/1471-2180/4/14en_GB
dc.rightsThis is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.en_GB
dc.subjectBase Sequenceen_GB
dc.subjectCarbohydrate Metabolismen_GB
dc.subjectComputational Biologyen_GB
dc.subjectConserved Sequenceen_GB
dc.subjectDNA Repairen_GB
dc.subjectDNA Replicationen_GB
dc.subjectDNA, Bacterialen_GB
dc.subjectGenome, Bacterialen_GB
dc.subjectMolecular Sequence Dataen_GB
dc.subjectNucleic Acid Conformationen_GB
dc.subjectPolysaccharidesen_GB
dc.subjectSequence Analysis, DNAen_GB
dc.subjectStreptomycesen_GB
dc.titleBioinformatic identification of novel regulatory DNA sequence motifs in Streptomyces coelicolor.en_GB
dc.typeArticleen_GB
dc.date.available2015-06-12T15:14:36Z
dc.identifier.issn1471-2180
exeter.place-of-publicationEngland
dc.descriptionJournal Articleen_GB
dc.descriptionResearch Support, Non-U.S. Gov'ten_GB
dc.descriptionCopyright © 2004 Studholme et al; licensee BioMed Central Ltd.en_GB
dc.identifier.journalBMC Microbiologyen_GB


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