Show simple item record

dc.contributor.authorTaniguchi, Kazuto
dc.contributor.authorRussell, Mark A.
dc.contributor.authorRichardson, Sarah J.
dc.contributor.authorMorgan, Noel G.
dc.date.accessioned2015-07-28T08:34:25Z
dc.date.issued2015-06-09
dc.description.abstractAIMS/HYPOTHESIS: In humans, the rate of beta cell proliferation declines rapidly during the postnatal period and remains low throughout adult life. Recent studies suggest that this may reflect the distribution of cell cycle regulators which, unusually, are located in the cytosolic compartment of beta cells in islets isolated from adults. In the present work, we examined whether the localisation of cyclin-D molecules is also cytosolic in the islet cells of pancreatic samples studied in situ. METHODS: Immunohistochemical approaches were employed to examine the subcellular localisation of cyclin-D1, -D2 and -D3 in human pancreatic samples recovered either from heart-beating donors or post mortem. Immunofluorescence methods were used to reveal the cellular localisation of cyclin-D1 and -D3. RESULTS: The distribution of cyclin-D2 was invariably cytosolic in islet cells, whereas the localisation of cyclin-D1 and -D3 varied according to the status of the donor. In pancreatic sections from heart-beating donors these molecules were primarily nuclear. By contrast, in samples collected post mortem, they were mainly cytosolic. Cyclin-D1 was detected only in beta cells whereas cyclin-D3 was detected in both alpha and beta cells. The proportion of donors who were immunopositive for cyclin-D1 declined from 71% in controls to 30% in those with type 1 diabetes. Cyclin-D3 was present in the islets of the majority of donors in both groups. CONCLUSIONS/INTERPRETATION: The subcellular localisation of cyclin-D molecules varies according to the status of the donor. Both cyclin-D1 and -D3 can be found in the nuclei of human islet cells in situ.en_GB
dc.description.sponsorshipEuropean Union’s Seventh Framework Programme PEVNET [FP7/2007-2013]en_GB
dc.description.sponsorshipEuropean Foundation for the Study of Diabetes (EFSD)/Lilly Fellowshipen_GB
dc.description.sponsorshipJuvenile Diabetes Research Foundation (JDRF) Career Development Awarden_GB
dc.description.sponsorshipJDRF Internationalen_GB
dc.identifier.citationVol. 58 (9), pp 2056-2063
dc.identifier.doi10.1007/s00125-015-3645-1
dc.identifier.grantnumber261441en_GB
dc.identifier.urihttp://hdl.handle.net/10871/17963
dc.language.isoenen_GB
dc.publisherSpringer Verlagen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/26055066en_GB
dc.rights.embargoreasonPublisher policyen_GB
dc.subjectBeta cellen_GB
dc.subjectCell cycleen_GB
dc.subjectCyclin-D moleculesen_GB
dc.subjectImmunofluorescence stainingen_GB
dc.subjectImmunohistochemistryen_GB
dc.subjectProliferationen_GB
dc.subjectType 1 diabetesen_GB
dc.titleThe subcellular distribution of cyclin-D1 and cyclin-D3 within human islet cells varies according to the status of the pancreas donoren_GB
dc.typeArticleen_GB
dc.identifier.issn0012-186X
dc.descriptionThe final publication is available at Springer via http://dx.doi.org/10.1007/s00125-015-3645-1en_GB
dc.identifier.eissn1432-0428
dc.identifier.journalDiabetologiaen_GB


Files in this item

This item appears in the following Collection(s)

Show simple item record