dc.contributor.author | Willcox, A | |
dc.contributor.author | Richardson, Sarah J. | |
dc.contributor.author | Bone, AJ | |
dc.contributor.author | Foulis, Alan K. | |
dc.contributor.author | Morgan, Noel G. | |
dc.date.accessioned | 2015-08-20T09:11:52Z | |
dc.date.issued | 2011-09 | |
dc.description.abstract | AIMS/HYPOTHESIS: The enteroviral capsid protein, VP1, was recently shown to be present in some beta cells in more than 60% of patients with recent-onset type 1 diabetes but in very few age-matched controls. The rate of proliferation of islet cells was also markedly increased in the type 1 diabetic patients. As it has been suggested that enteroviruses replicate most efficiently in proliferating cells, we have investigated whether VP1 is preferentially present in proliferating beta cells in type 1 diabetes. METHODS: Combined immunoperoxidase and immunofluorescence staining was used to record the presence of enteroviral VP1, insulin and Ki67 in the islets of recent-onset type 1 diabetic patients. RESULTS: From a total of 1,175 islets, 359 (30.5%) contained insulin. VP1-producing endocrine cells were found in 72 islets (6.1% of total), all of which retained insulin. Ki67(+) endocrine cells were present in 52 (4.4%) islets, with 44 (84.6%) of these being insulin-positive. Overall, 28 of 1,175 (2.4%) islets contained both Ki67(+) cells and VP1(+) cells. Dual positivity of these markers accounted for 38.9% of the total VP1(+) islets and 53.8% of the total Ki67(+) islets. No individual islet cells were dual-positive for Ki67 and VP1. CONCLUSIONS/INTERPRETATION: Ki67(+) cells were frequently observed in islets that also contained VP1(+) cells, suggesting that the factors facilitating viral replication may also drive islet cell proliferation. However, in an individual cell, VP1 production does not require concurrent beta cell proliferation. | en_GB |
dc.description.sponsorship | Juvenile Diabetes Research Foundation | en_GB |
dc.description.sponsorship | Diabetes Research and Wellness Foundation | en_GB |
dc.identifier.citation | Vol. 54, pp. 2417 - 2420 | en_GB |
dc.identifier.doi | 10.1007/s00125-011-2192-7 | |
dc.identifier.uri | http://hdl.handle.net/10871/18088 | |
dc.language.iso | en | en_GB |
dc.publisher | Springer | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/21597997 | en_GB |
dc.subject | Adolescent | en_GB |
dc.subject | Adult | en_GB |
dc.subject | Capsid Proteins | en_GB |
dc.subject | Cell Proliferation | en_GB |
dc.subject | Child | en_GB |
dc.subject | Diabetes Mellitus, Type 1 | en_GB |
dc.subject | Enterovirus | en_GB |
dc.subject | Female | en_GB |
dc.subject | Humans | en_GB |
dc.subject | Immunohistochemistry | en_GB |
dc.subject | Infant | en_GB |
dc.subject | Insulin | en_GB |
dc.subject | Insulin-Secreting Cells | en_GB |
dc.subject | Islets of Langerhans | en_GB |
dc.subject | Ki-67 Antigen | en_GB |
dc.subject | Male | en_GB |
dc.subject | Retrospective Studies | en_GB |
dc.subject | Virus Replication | en_GB |
dc.subject | Young Adult | en_GB |
dc.title | Immunohistochemical analysis of the relationship between islet cell proliferation and the production of the enteroviral capsid protein, VP1, in the islets of patients with recent-onset type 1 diabetes. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2015-08-20T09:11:52Z | |
dc.identifier.issn | 0012-186X | |
exeter.place-of-publication | Germany | |
dc.description | Author version of article submitted to Diabetologia The final publication is available at Springer via http://dx.doi.org/10.1007/s00125-011-2192-7 | en_GB |
dc.identifier.eissn | 1432-0428 | |
dc.identifier.journal | Diabetologia | en_GB |
dc.identifier.pmid | 21597997 | |