dc.contributor.author | de Bruyn, M | |
dc.contributor.author | Wiersma, VR | |
dc.contributor.author | Wouters, MC | |
dc.contributor.author | Samplonius, DF | |
dc.contributor.author | Klip, HG | |
dc.contributor.author | Helfrich, W | |
dc.contributor.author | Nijman, HW | |
dc.contributor.author | Eggleton, P | |
dc.contributor.author | Bremer, E | |
dc.date.accessioned | 2015-09-14T07:51:11Z | |
dc.date.issued | 2015-04 | |
dc.description.abstract | Recently, a small subset of T cells that expresses the B cell marker CD20 has been identified in healthy volunteers and in patients with rheumatoid arthritis and multiple sclerosis. The origin of these CD20-positive T cells as well as their relevance in human disease remains unclear. Here, we identified that after functional B cell/T cell interaction CD20 molecules are transferred to the cell surface of T cells by trogocytosis together with the established trogocytosis marker HLA-DR. Further, the presence of CD20 on isolated CD20(+) T cells remained stable for up to 48h of ex vivo culture. These CD20(+) T cells almost exclusively produced IFNγ (∼70% vs. ∼20% in the CD20(-) T cell population) and were predominantly (CD8(+)) effector memory T cells (∼60-70%). This IFNγ producing and effector memory phenotype was also determined for CD20(+) T cells as detected in the peripheral blood and ascitic fluids of ovarian cancer (OC) patients. In the latter, the percentage of CD20(+) T cells was further strongly increased (from ∼6% in peripheral blood to 23% in ascitic fluid). Taken together, the data presented here indicate that CD20 is transferred to T cells upon intimate T cell/B cell interaction. Further, CD20(+) T cells are of memory and IFNγ producing phenotype and are present in increased amounts in ascitic fluid of OC patients. | en_GB |
dc.description.sponsorship | Netherlands Organization for Scientific Research | en_GB |
dc.description.sponsorship | Dutch Cancer Society | en_GB |
dc.description.sponsorship | Alexander von Humboldt Foundation | en_GB |
dc.identifier.citation | Vol. 4, Iss. 4, pp. e999536 - | en_GB |
dc.identifier.doi | 10.1080/2162402X.2014.999536 | |
dc.identifier.other | 999536 | |
dc.identifier.uri | http://hdl.handle.net/10871/18224 | |
dc.language.iso | en | en_GB |
dc.publisher | Taylor & Francis | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/26137418 | en_GB |
dc.relation.url | http://www.tandfonline.com/doi/full/10.1080/2162402X.2014.999536 | en_GB |
dc.rights.embargoreason | Publisher's policy | en_GB |
dc.rights | This is an Accepted Manuscript of an article published by Taylor & Francis in OncoImmunology on 19 March 2015, available online: http://wwww.tandfonline.com/10.1080/2162402X.2014.999536#sthash.vdH7nh5C.dpuf. | en_GB |
dc.subject | APC, Antigen-Presenting Cell | en_GB |
dc.subject | Ascites | en_GB |
dc.subject | CD20 | en_GB |
dc.subject | CTL, Cytotoxic T Lymphocyte | en_GB |
dc.subject | FSC, Forward Scatter | en_GB |
dc.subject | OC, Ovarian Cancer | en_GB |
dc.subject | PBMC, Peripheral Blood Mononuclear Cell | en_GB |
dc.subject | Regulatory T cell | en_GB |
dc.subject | SCC, Side Scatter | en_GB |
dc.subject | TC, Cytotoxic T cell | en_GB |
dc.subject | TCM, Central Memory T cell | en_GB |
dc.subject | TEM, Effector Memory T cell | en_GB |
dc.subject | TH, Helper T cell | en_GB |
dc.subject | TIL, Tumor Infiltrating T cell | en_GB |
dc.subject | TNaïve, Naïve T cell | en_GB |
dc.subject | TTD, Terminally Differentiated T cell | en_GB |
dc.subject | Treg | en_GB |
dc.subject | cancer immunology | en_GB |
dc.subject | ovarian cancer | en_GB |
dc.subject | trogocytosis | en_GB |
dc.title | CD20(+) T cells have a predominantly Tc1 effector memory phenotype and are expanded in the ascites of patients with ovarian cancer. | en_GB |
dc.type | Article | en_GB |
dc.identifier.issn | 2162-4011 | |
dc.description | Copyright © 2015 Taylor & Francis Group, LLC | en_GB |
dc.identifier.journal | Oncoimmunology | en_GB |