| dc.contributor.author | McGhie, EJ | |
| dc.contributor.author | Isupov, MN | |
| dc.contributor.author | Schröder, E | |
| dc.contributor.author | Littlechild, JA | |
| dc.date.accessioned | 2015-10-08T09:04:25Z | |
| dc.date.issued | 1998-09-01 | |
| dc.description.abstract | The oxygenating constituent of the 3,6-diketocamphane monooxygenase isozyme from Pseudomonas putida NCIMB 10007 has been crystallized under two different conditions. Crystals were initially grown from polyethylene glycol (PEG) 8000 and sodium acetate using the vapour-phase diffusion method. The crystals were of orthorhombic P212121 space group, with cell dimensions a = 55.8, b = 94.5 and c = 163.7 A and diffracted to 2.8 A resolution. More recently, improved crystals, which diffracted beyond 2 A, have been grown from ammonium sulfate. These crystals also belong to the orthorhombic P212121 space group, with cell dimensions of a = 54.6, b = 93.2 and c = 154. 1 A. A full native data set to 2.5 A resolution has been collected from the ammonium sulfate grown crystals. | en_GB |
| dc.description.sponsorship | BBSRC | en_GB |
| dc.identifier.citation | Vol. 54, Iss.5, pp. 1035 - 1038 | en_GB |
| dc.identifier.doi | 10.1107/S0907444998004946 | |
| dc.identifier.uri | http://hdl.handle.net/10871/18388 | |
| dc.language.iso | en | en_GB |
| dc.publisher | International Union of Crystallography | en_GB |
| dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/9757131 | en_GB |
| dc.relation.url | http://onlinelibrary.wiley.com/doi/10.1107/S0907444998004946/abstract | en_GB |
| dc.rights | Copyright © 1998 International Union of Crystallography | en_GB |
| dc.subject | Bacterial Proteins | en_GB |
| dc.subject | Camphor | en_GB |
| dc.subject | Crystallization | en_GB |
| dc.subject | Crystallography, X-Ray | en_GB |
| dc.subject | Oxygenases | en_GB |
| dc.subject | Protein Conformation | en_GB |
| dc.subject | Pseudomonas putida | en_GB |
| dc.title | Crystallization and preliminary X-ray diffraction studies of the oxygenating subunit of 3,6-diketocamphane monooxygenase from Pseudomonas putida. | en_GB |
| dc.type | Article | en_GB |
| dc.date.available | 2015-10-08T09:04:25Z | |
| dc.identifier.issn | 0907-4449 | |
| exeter.place-of-publication | DENMARK | |
| dc.description | Journal Article | en_GB |
| dc.description | Research Support, Non-U.S. Gov't | en_GB |
| dc.identifier.journal | Acta Crystallographica Section D: Biological Crystallography | en_GB |
