dc.contributor.author | Bourne, PC | |
dc.contributor.author | Isupov, MN | |
dc.contributor.author | Littlechild, JA | |
dc.date.accessioned | 2015-10-08T09:19:26Z | |
dc.date.issued | 1999-04 | |
dc.description.abstract | A novel bacterial esterase has been crystallized in two forms suitable for X-ray diffraction studies. Crystals have been obtained by vapour-phase diffusion at 290 K using ammonium sulfate as precipitant. The first crystals grew in space group C2 with unit-cell parameters a = 134.7, b = 55.8, c = 110.3 A, beta = 125.1 degrees. A monoclinic data set has been collected to 2.0 A resolution. Microseeding yielded a second crystal form which grew in space group P212121 with unit-cell parameters a = 57.1, b = 115.4, c = 130.4 A. Native data from these crystals have been collected to 1.6 A resolution. A molecular envelope has been determined using an uranyl acetate derivative for phase calculation. | en_GB |
dc.description.sponsorship | BBSRC | en_GB |
dc.description.sponsorship | SmithKline Beecham Pharmaceuticals | en_GB |
dc.description.sponsorship | European Union | en_GB |
dc.identifier.citation | Vol. 55, Iss. 4, pp. 915 - 917 | en_GB |
dc.identifier.doi | 10.1107/S0907444998018459 | |
dc.identifier.grantnumber | CHGE-CT93-0040 | en_GB |
dc.identifier.other | AD0057 | |
dc.identifier.uri | http://hdl.handle.net/10871/18391 | |
dc.language.iso | en | en_GB |
dc.publisher | International Union of Crystallography | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/10089333 | en_GB |
dc.relation.url | http://onlinelibrary.wiley.com/doi/10.1107/S0907444998018459/abstract | en_GB |
dc.rights | Copyright © 1999 International Union of Crystallography | en_GB |
dc.subject | Bacterial Proteins | en_GB |
dc.subject | Crystallization | en_GB |
dc.subject | Esterases | en_GB |
dc.subject | Rhizobium | en_GB |
dc.subject | Solvents | en_GB |
dc.subject | X-Ray Diffraction | en_GB |
dc.title | Crystallization and preliminary x-ray diffraction studies of a novel bacterial esterase. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2015-10-08T09:19:26Z | |
dc.identifier.issn | 0907-4449 | |
exeter.place-of-publication | DENMARK | |
dc.description | Journal Article | en_GB |
dc.description | Research Support, Non-U.S. Gov't | en_GB |
dc.identifier.journal | Acta Crystallographica Section D: Biological Crystallography | en_GB |