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Characterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.

dc.contributor.authorFleming, TM
dc.contributor.authorJones, CE
dc.contributor.authorPiper, PW
dc.contributor.authorCowan, DA
dc.contributor.authorIsupov, MN
dc.contributor.authorLittlechild, JA
dc.date.accessioned2015-10-08T10:03:37Z
dc.date.issued1998-07-01
dc.description.abstractRecombinant Sulfolobus solfataricus glyceraldehyde-3-phosphate dehydrogenase has been purified and found to be a tetramer of 148 kDa. The enzyme shows dual cofactor specificity and uses NADP+ in preference to NAD+. The sequence has been compared with other GAPDH proteins including those from other archaeal sources. The purified protein has been crystallized from ammonium sulfate to produce crystals that diffract to 2.4 A with a space group of P43212 or P41212. A native data set has been collected to 2.4 A using synchrotron radiation and cryocooling.en_GB
dc.description.sponsorshipEuropean Unionen_GB
dc.description.sponsorshipBBSRCen_GB
dc.identifier.citationVol. 54, Iss.4, pp. 671 - 674en_GB
dc.identifier.doi10.1107/S0907444997018076
dc.identifier.grantnumberCHGE-CT93±0040en_GB
dc.identifier.urihttp://hdl.handle.net/10871/18397
dc.language.isoenen_GB
dc.publisherInternational Union of Crystallographyen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/9761871en_GB
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1107/S0907444997018076/abstracten_GB
dc.rightsCopyright © 1998 International Union of Crystallographyen_GB
dc.subjectAmino Acid Sequenceen_GB
dc.subjectBacterial Proteinsen_GB
dc.subjectCrystallizationen_GB
dc.subjectCrystallography, X-Rayen_GB
dc.subjectGlyceraldehyde-3-Phosphate Dehydrogenasesen_GB
dc.subjectMass Spectrometryen_GB
dc.subjectMolecular Sequence Dataen_GB
dc.subjectProtein Conformationen_GB
dc.subjectRecombinant Fusion Proteinsen_GB
dc.subjectSequence Alignmenten_GB
dc.subjectSequence Analysisen_GB
dc.subjectSequence Homology, Amino Aciden_GB
dc.subjectSulfolobusen_GB
dc.titleCharacterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.en_GB
dc.typeArticleen_GB
dc.date.available2015-10-08T10:03:37Z
dc.identifier.issn0907-4449
exeter.place-of-publicationDENMARK
dc.descriptionComparative Studyen_GB
dc.descriptionJournal Articleen_GB
dc.descriptionResearch Support, Non-U.S. Gov'ten_GB
dc.identifier.journalActa Crystallographica Section D: Biological Crystallographyen_GB


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