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Crystallization and preliminary X-ray analysis of a gamma-lactamase.

dc.contributor.authorGonsalvez, IS
dc.contributor.authorIsupov, MN
dc.contributor.authorLittlechild, JA
dc.date.accessioned2015-10-08T10:18:12Z
dc.date.issued2001-02
dc.description.abstractAn enzyme from Comomonas acidovorans has been isolated that is specific for the stereospecific hydrolysis of (+)gamma-lactam. This so-called (+)gamma-lactamase has important applications in biotransformation reactions. The enzyme has been crystallized by vapour-phase diffusion using polyethylene glycol 4000 as a precipitant. Addition of a detergent, beta-octylglucoside, was found to be essential for obtaining diffraction-quality crystals. The crystals grow in the space group P1, with unit-cell parameters a = 63.0, b = 93.2, c = 152.4 A, alpha = 104.3, beta = 92.6, gamma = 108.5 degrees, and diffract to 2 A resolution using synchrotron radiation. Native data from these crystals have been collected to 2.4 A.en_GB
dc.description.sponsorshipBBSRCen_GB
dc.description.sponsorshipEuropean Union (HCMP Access to Large Installations Project)en_GB
dc.description.sponsorshipUniversity of Exeteren_GB
dc.identifier.citationVol. 57, Iss.2, pp. 284 - 286en_GB
dc.identifier.doi10.1107/S0907444900016838
dc.identifier.grantnumberCHGE-CT93-0040en_GB
dc.identifier.otherS0907444900016838
dc.identifier.urihttp://hdl.handle.net/10871/18400
dc.language.isoenen_GB
dc.publisherInternational Union of Crystallographyen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/11173481en_GB
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1107/S0907444900016838/abstracten_GB
dc.rightsCopyright © 2001 International Union of Crystallographyen_GB
dc.subjectAmidohydrolasesen_GB
dc.subjectBetaproteobacteriaen_GB
dc.subjectCrystallizationen_GB
dc.subjectCrystallography, X-Rayen_GB
dc.subjectEnzyme Inhibitorsen_GB
dc.subjectMass Spectrometryen_GB
dc.subjectPolyethylene Glycolsen_GB
dc.subjectSequence Alignmenten_GB
dc.subjectSequence Homology, Amino Aciden_GB
dc.titleCrystallization and preliminary X-ray analysis of a gamma-lactamase.en_GB
dc.typeArticleen_GB
dc.date.available2015-10-08T10:18:12Z
dc.identifier.issn0907-4449
exeter.place-of-publicationDenmark
dc.descriptionComparative Studyen_GB
dc.descriptionJournal Articleen_GB
dc.descriptionResearch Support, Non-U.S. Gov'ten_GB
dc.identifier.journalActa Crystallographica Section D: Biological Crystallographyen_GB


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