Development of molecular markers tightly linked to Pvr4 gene in pepper using next-generation sequencing.
Studholme, David J.
It is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. Potyvirus resistance 4 (Pvr4) locus in pepper confers resistance to three pathotypes of potato virus Y and to pepper mottle virus. We describe the use of next-generation sequencing technology to generate molecular markers tightly linked to Pvr4. Initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate PCR-based markers and map Pvr4. Subsequently, the genomic sequence of pepper was used, and more than 5000 single-nucleotide variants (SNVs) were identified within the interval. In addition, we identified nucleotide binding site-leucine-rich repeat-type disease resistance genes within the interval. Several of these SNVs were converted to molecular markers desirable for large-scale molecular breeding programmes.
Copyright The Author(s) 2015. This article is published with open access at Springerlink.com. The online version of this article (doi:10.1007/s11032-015-0294-5) contains supplementary material, which is available to authorized users.
Vol. 35, p.101 (9 pages)