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dc.contributor.authorCurnow, Alison
dc.contributor.authorHaller, JC
dc.contributor.authorBown, SG
dc.date.accessioned2016-01-11T11:07:19Z
dc.date.issued2000-11
dc.description.abstractCurrently, the clinical use of 5-aminolaevulinic acid (ALA) induced protoporphyrin IX (PPIX) for photodynamic therapy (PDT) is limited by the maximum tolerated oral ALA dose (60 mg/kg). Attempts have been made to enhance this treatment modality without increasing the administered dose of ALA. One way to do this is through light dose fractionation, where the irradiation is interrupted at a particular point for a short period of time. This can produce up to three times more necrosis than with the same light dose delivered without a break. An oxygen microelectrode was employed to study the effect of continuous and fractionated light regimes on the level of oxygen in the colon of normal Wistar rats during ALA PDT. A rapid decline in pO2 occurred close to the irradiation fibre as soon as the light dose commenced. With the fractionated regime, a partial recovery in pO2 was observed during the dark interval which was reversed soon after the second light fraction commenced. We have shown that the level of tissue oxygen at the treatment site is affected differently when the light dose is fractionated, than when continuous illumination is employed. This factor may at least partially explain the difference in outcome of these two treatment regimes. Further, oxygen measurements might prove to be a useful way of monitoring PDT treatments if they can predict whether tissue is likely to be viable following treatment.en_GB
dc.identifier.citationVol. 58, pp. 149 - 155en_GB
dc.identifier.doi10.1016/S1011-1344(00)00120-2
dc.identifier.urihttp://hdl.handle.net/10871/19197
dc.language.isoenen_GB
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/11233643en_GB
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S1011134400001202en_GB
dc.subjectAminolevulinic Aciden_GB
dc.subjectAnimalsen_GB
dc.subjectColonen_GB
dc.subjectFemaleen_GB
dc.subjectLighten_GB
dc.subjectMonitoring, Physiologicen_GB
dc.subjectNecrosisen_GB
dc.subjectOxygenen_GB
dc.subjectPhotobiologyen_GB
dc.subjectPhotochemotherapyen_GB
dc.subjectProtoporphyrinsen_GB
dc.subjectRatsen_GB
dc.subjectRats, Wistaren_GB
dc.titleOxygen monitoring during 5-aminolaevulinic acid induced photodynamic therapy in normal rat colon: comparison of continuous and fractionated light regimesen_GB
dc.typeArticleen_GB
dc.date.available2016-01-11T11:07:19Z
dc.identifier.issn1011-1344
exeter.place-of-publicationSwitzerland
dc.descriptionComparative Studyen_GB
dc.descriptionJournal Articleen_GB
dc.descriptionResearch Support, Non-U.S. Gov'ten_GB
dc.descriptionAuthor's post-print is subject to a Creative Commons Attribution Non-Commercial No Derivatives Licenseen_GB
dc.identifier.eissn1873-2682
dc.identifier.journalJournal of Photochemistry and Photobiology B: Biologyen_GB


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