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dc.contributor.authorHannon, E
dc.contributor.authorLunnon, Katie
dc.contributor.authorSchalkwyk, Leonard
dc.contributor.authorMill, J
dc.date.accessioned2016-01-13T13:31:34Z
dc.date.issued2015-10-12
dc.description.abstractGiven the tissue-specific nature of epigenetic processes, the assessment of disease-relevant tissue is an important consideration for epigenome-wide association studies (EWAS). Little is known about whether easily accessible tissues, such as whole blood, can be used to address questions about interindividual epigenomic variation in inaccessible tissues, such as the brain. We quantified DNA methylation in matched DNA samples isolated from whole blood and 4 brain regions (prefrontal cortex, entorhinal cortex, superior temporal gyrus, and cerebellum) from 122 individuals. We explored co-variation between tissues and the extent to which methylomic variation in blood is predictive of interindividual variation identified in the brain. For the majority of DNA methylation sites, interindividual variation in whole blood is not a strong predictor of interindividual variation in the brain, although the relationship with cortical regions is stronger than with the cerebellum. Variation at a subset of probes is strongly correlated across tissues, even in instances when the actual level of DNA methylation is significantly different between them. A substantial proportion of this co-variation, however, is likely to result from genetic influences. Our data suggest that for the majority of the genome, a blood-based EWAS for disorders where brain is presumed to be the primary tissue of interest will give limited information relating to underlying pathological processes. These results do not, however, discount the utility of using a blood-based EWAS to identify biomarkers of disease phenotypes manifest in the brain. We have generated a searchable database for the interpretation of data from blood-based EWAS analyses ( http://epigenetics.essex.ac.uk/bloodbrain/ ).en_GB
dc.description.sponsorshipAlzheimer's Research UKen_GB
dc.description.sponsorshipUS National Institutes of Healthen_GB
dc.description.sponsorshipMedical Research Council (MRC)en_GB
dc.identifier.citationVol. 10 (11), pp. 1024 - 1032en_GB
dc.identifier.doi10.1080/15592294.2015.1100786
dc.identifier.grantnumberR01 AG036039en_GB
dc.identifier.grantnumberMR/K013807/1en_GB
dc.identifier.urihttp://hdl.handle.net/10871/19225
dc.language.isoenen_GB
dc.publisherTaylor & Francis for Epigenetics Societyen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/26457534en_GB
dc.rights© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC© Eilis Hannon, Katie Lunnon, Leonard Schalkwyk and Jonathan Mill This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. Permission is granted subject to the terms of the License under which the work was published. Please check the License conditions for the work which you wish to reuse. Full and appropriate attribution must be given. This permission does not cover any third party copyrighted material which may appear in the work requested.
dc.subjectDNA methylationen_GB
dc.subjectIllumina 450K arrayen_GB
dc.subjectblooden_GB
dc.subjectbrainen_GB
dc.subjectcerebellumen_GB
dc.subjectcortexen_GB
dc.subjectepigenetic epidemiologyen_GB
dc.titleInterindividual methylomic variation across blood, cortex, and cerebellum: implications for epigenetic studies of neurological and neuropsychiatric phenotypesen_GB
dc.typeArticleen_GB
dc.identifier.issn1559-2294
exeter.place-of-publicationUnited States
dc.descriptionCopyright © 2015 Taylor & Francis This is an Accepted Manuscript of an article published by Taylor & Francis in Epigenetics on 12 October 2015 available online: http://www.tandfonline.com/10.1080/15592294.2015.1100786en_GB
dc.identifier.eissn1559-2308
dc.identifier.journalEpigeneticsen_GB


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