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dc.contributor.authorMorrison, F
dc.contributor.authorJohnstone, K
dc.contributor.authorMurray, A
dc.contributor.authorLocke, J
dc.contributor.authorHarries, LW
dc.date.accessioned2016-02-12T08:56:04Z
dc.date.issued2012
dc.description.abstractAltered expression of oxidative metabolism genes has been described in the skeletal muscle of individuals with type 2 diabetes. Pancreatic beta cells contain low levels of antioxidant enzymes and are particularly susceptible to oxidative stress. In this study, we explored the effect of hyperglycemia-induced oxidative stress on a panel of oxidative metabolism genes in a rodent beta cell line. We exposed INS-1 rodent beta cells to low (5.6 mmol/L), ambient (11 mmol/L), and high (28 mmol/L) glucose conditions for 48 hours. Increases in oxidative stress were measured using the fluorescent probe dihydrorhodamine 123. We then measured the expression levels of a panel of 90 oxidative metabolism genes by real-time PCR. Elevated reactive oxygen species (ROS) production was evident in INS-1 cells after 48 hours (P < 0.05). TLDA analysis revealed a significant (P < 0.05) upregulation of 16 of the 90 genes under hyperglycemic conditions, although these expression differences did not reflect differences in ROS. We conclude that although altered glycemia may influence the expression of some oxidative metabolism genes, this effect is probably not mediated by increased ROS production. The alterations to the expression of oxidative metabolism genes previously observed in human diabetic skeletal muscle do not appear to be mirrored in rodent pancreatic beta cells.en_GB
dc.description.sponsorshipWellcome Trusten_GB
dc.description.sponsorshipMendip Golf Cluben_GB
dc.identifier.citationVol. 2012, pp. 793783 -en_GB
dc.identifier.doi10.1155/2012/793783
dc.identifier.grantnumberWT081278MAen_GB
dc.identifier.urihttp://hdl.handle.net/10871/19747
dc.language.isoenen_GB
dc.publisherHindawi Publishing Corporationen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/22454629en_GB
dc.relation.urlhttp://www.hindawi.com/journals/jdr/2012/793783/en_GB
dc.rightsCopyright © 2012 Faer Morrison et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_GB
dc.subjectAnimalsen_GB
dc.subjectCell Lineen_GB
dc.subjectCells, Cultureden_GB
dc.subjectGene Expressionen_GB
dc.subjectInsulin-Secreting Cellsen_GB
dc.subjectOxidation-Reductionen_GB
dc.subjectOxidative Stressen_GB
dc.subjectRatsen_GB
dc.subjectReactive Oxygen Speciesen_GB
dc.titleOxidative metabolism genes are not responsive to oxidative stress in rodent Beta cell lines.en_GB
dc.typeArticleen_GB
dc.date.available2016-02-12T08:56:04Z
dc.identifier.issn1687-5214
exeter.place-of-publicationUnited States
dc.descriptionPublisheden_GB
dc.descriptionJournal Articleen_GB
dc.descriptionResearch Support, Non-U.S. Gov'ten_GB
dc.identifier.eissn1687-5303
dc.identifier.journalExperimental Diabetes Researchen_GB


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