dc.contributor.author | Alptekin, S | |
dc.contributor.author | Bass, C | |
dc.contributor.author | Nicholls, C | |
dc.contributor.author | Paine, MJ | |
dc.contributor.author | Clark, SJ | |
dc.contributor.author | Field, L | |
dc.contributor.author | Moores, GD | |
dc.date.accessioned | 2016-02-19T14:10:37Z | |
dc.date.issued | 2016-01-20 | |
dc.description.abstract | Honey bees, Apis mellifera, are markedly less sensitive to neonicotinoid insecticides containing a cyanoimino pharmacophore than to those with a nitroimino group. Although previous work has suggested that this results from enhanced metabolism of the former by detoxification enzymes, the specific enzyme(s) involved remain to be characterized. In this work, a pretreatment of honey bees with a sublethal dose of thiacloprid resulted in induced insensitivity to the same compound immediately following thiacloprid feeding. A longer pretreatment time resulted in no, or increased, sensitivity. Transcriptome profiling, using microarrays, identified a number of genes encoding detoxification enzymes that were over-expressed significantly in insecticide-treated bees compared with untreated controls. These included five P450s, CYP6BE1, CYP305D1, CYP6AS5, CYP315A1, CYP301A1, and a carboxyl/cholinesterase (CCE) CCE8. Four of these P450s were functionally expressed in Escherichia coli and their ability to metabolize thiacloprid examined by liquid chromatography-mass spectrometry (LC-MS) analysis. | en_GB |
dc.description.sponsorship | AHDB cereals and Oilseeds | en_GB |
dc.description.sponsorship | Agriculture and Horticulture Development Board, UK | en_GB |
dc.description.sponsorship | BBSRC | en_GB |
dc.identifier.citation | First published online: 20 JAN 2016 | en_GB |
dc.identifier.doi | 10.1111/imb.12211 | |
dc.identifier.uri | http://hdl.handle.net/10871/19971 | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/26790026 | en_GB |
dc.relation.url | http://onlinelibrary.wiley.com/doi/10.1111/imb.12211/abstract | en_GB |
dc.rights.embargoreason | Publisher's policy. | en_GB |
dc.rights | Copyright © 2016 The Royal Entomological Society. This is the peer reviewed version of the following article: Alptekin, S., Bass, C., Nicholls, C., Paine, M. J. I., Clark, S. J., Field, L. and Moores, G. D. (2016), Induced thiacloprid insensitivity in honeybees (Apis mellifera L.) is associated with up-regulation of detoxification genes. Insect Molecular Biology. doi: 10.1111/imb.12211, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/imb.12211/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | en_GB |
dc.subject | Apis mellifera | en_GB |
dc.subject | P450 | en_GB |
dc.subject | esterase | en_GB |
dc.subject | induction | en_GB |
dc.subject | metabolism | en_GB |
dc.subject | thiacloprid | en_GB |
dc.title | Induced thiacloprid insensitivity in honeybees (Apis mellifera L.) is associated with up-regulation of detoxification genes. | en_GB |
dc.type | Article | en_GB |
dc.identifier.issn | 0962-1075 | |
dc.description | Published online | en_GB |
dc.description | JOURNAL ARTICLE | en_GB |
dc.identifier.eissn | 1365-2583 | |
dc.identifier.journal | Insect Molecular Biology | en_GB |