dc.contributor.author | Marut, W | |
dc.contributor.author | Jamier, V | |
dc.contributor.author | Kavian, N | |
dc.contributor.author | Servettaz, A | |
dc.contributor.author | Eggleton, P | |
dc.contributor.author | Winyard, PG | |
dc.contributor.author | Anwar, A | |
dc.contributor.author | Nicco, C | |
dc.contributor.author | Jacob, C | |
dc.contributor.author | Chéreau, C | |
dc.contributor.author | Weill, B | |
dc.contributor.author | Batteux, F | |
dc.date.accessioned | 2016-02-29T15:23:34Z | |
dc.date.issued | 2013-10-28 | |
dc.description.abstract | Introduction: The aim of this study was to test the naturally occurring organosulfur compound dipropyltetrasulfide (DPTTS) found in plants, which has antibiotic and anti-cancer properties, as a treatment of HOCl-induced systemic sclerosis in the mouse. Methods: The pro-oxidative, anti-proliferative and cytotoxic effects of DPTTS were evaluated ex vivo on fibroblasts from normal and HOCl-mice. In vivo, the anti-fibrotic and immunomodulating properties of DPTTS were evaluated in the skin and lungs of HOCl-mice. Results: H2O2 production was higher in fibroblasts derived from HOCl-mice than in normal fibroblasts (P<0.05). DPTTS did not increase H2O2 production in normal fibroblasts, but DPTTS dose-dependently increased H2O2 production in HOCl-fibroblasts (P<0.001 with 40μM DPTTS). Because H2O2 reached a lethal threshold in cells from HOCl-mice, the anti-proliferative, cytotoxic and pro-apoptotic effects of DPTTS were significantly higher in HOCl-fibroblasts than for normal fibroblasts. In vivo, DPTTS decreased dermal thickness (P<0.001), collagen content in skin (P<0.01) and lungs (P<0.05), SMA (P<0.01) and pSMAD2/3 (P<0.01) expression in skin, formation of advanced oxidation protein products and anti-DNA topoisomerase-1 antibodies in serum (P<0.05) versus untreated HOCl- mice. Moreover, in HOCl-mice, DPTTS reduced splenic B cell counts (P<0.01), the proliferative rates of B-splenocytes stimulated by lipopolysaccharide (P<0.05) and T-splenocytes stimulated by anti-CD3/CD28 mAb (P<0.001). Ex vivo, it also reduced the production of IL-4 and IL-13 by activated T cells (P<0.05 in both cases). Conclusions: The natural organosulfur compound DPTTS prevents skin and lung fibrosis in the mouse through the selective killing of diseased fibroblasts and its immunomodulating properties. DPTTS may be a potential treatment of Systemic sclerosis. | en_GB |
dc.description.sponsorship | This work was supported by European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement 215009 RedCat for financial support. The authors are grateful to Ms Agnes for her excellent typing of the manuscript. | en_GB |
dc.identifier.citation | Vol. 15, Iss. 5, R167 | en_GB |
dc.identifier.doi | 10.1186/ar4351 | |
dc.identifier.uri | http://hdl.handle.net/10871/20265 | |
dc.language.iso | en | en_GB |
dc.publisher | Biomed Central | en_GB |
dc.relation.url | http://www.arthritis-research.com/content/15/5/R167 | en_GB |
dc.rights | This is the final version of the article. It first appeared from BioMed Central via http://dx.doi.org/10.1186/ar4351. | en_GB |
dc.title | The natural organosulfur compound dipropyltetrasulfide prevents HOCL-induced systemic sclerosis in the mouse | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2016-02-29T15:23:34Z | |
dc.contributor.editor | Maini, R | |
dc.contributor.editor | Lipsky, P | |
dc.identifier.issn | 1478-6354 | |
exeter.place-of-publication | UK | |
dc.description | Published | en_GB |
dc.description | Article | en_GB |
dc.identifier.eissn | 1478-6362 | |
dc.identifier.journal | Arthritis Research and Therapy | en_GB |