dc.contributor.author | Wiehr, S | |
dc.contributor.author | Warnke, P | |
dc.contributor.author | Rolle, A-M | |
dc.contributor.author | Schutz, M | |
dc.contributor.author | Kohlhofer, U | |
dc.contributor.author | Quintanilla-Martinez de Fend, L | |
dc.contributor.author | Maurer, A | |
dc.contributor.author | Thornton, C | |
dc.contributor.author | Boschetti, F | |
dc.contributor.author | Reischl, G | |
dc.contributor.author | Autenrieth, I | |
dc.contributor.author | Pichler, BJ | |
dc.contributor.author | Autenrieth, S | |
dc.date.accessioned | 2016-03-01T14:35:29Z | |
dc.date.issued | 2016-02-26 | |
dc.description.abstract | The specific and rapid detection of Enterobacteriaceae, the most frequent cause of gram-negative bacterial infections in humans, remains a major challenge. We developed a non-invasive method to rapidly detect systemic Yersinia enterocolitica infections using immunoPET (antibody-targeted positron emission tomography) with [64Cu]NODAGA-labeled Yersinia-specific polyclonal antibodies targeting the outer membrane protein YadA. In contrast to the tracer [18F]FDG, [64Cu]NODAGA-YadA uptake co-localized in a dose dependent manner with bacterial lesions of Yersinia-infected mice, as detected by magnetic resonance (MR) imaging. This was accompanied by elevated uptake of [64Cu]NODAGA-YadA in infected tissues, in ex vivo biodistribution studies, whereas reduced uptake was observed following blocking with unlabeled anti-YadA antibody. We show, for the first time, a bacteria-specific, antibody-based, in vivo imaging method for the diagnosis of a Gram-negative enterobacterial infection as a proof of concept, which may provide new insights into pathogen-host interactions. | en_GB |
dc.description.sponsorship | The research leading to these results has received funding from the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement n°602820, from the European Social Fund Baden-Württemberg (to SEA), and from the Deutsche Forschungsgemeinschaft (grant WI 3777/1-2; to SW). | en_GB |
dc.identifier.citation | Published online: February 26, 2016 | en_GB |
dc.identifier.doi | 10.18632/oncotarget.7770 | |
dc.identifier.uri | http://hdl.handle.net/10871/20337 | |
dc.language.iso | en | en_GB |
dc.publisher | Impact Journals | en_GB |
dc.relation.url | http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5b%5d=7770 | en_GB |
dc.rights | This is the final version of the article. It first appeared from Impact Journals via http://dx.doi.org/10.18632/oncotarget.7770. | en_GB |
dc.subject | bacteria | en_GB |
dc.subject | PET/MR | en_GB |
dc.subject | in vivo imaging | en_GB |
dc.subject | 64Cu | en_GB |
dc.subject | antibody | en_GB |
dc.subject | Immunology and Microbiology Section | en_GB |
dc.subject | Immune response | en_GB |
dc.subject | Immunity | en_GB |
dc.title | New pathogen-specific immunoPET/MR tracer for molecular imaging of a systemic bacterial infection | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2016-03-01T14:35:29Z | |
dc.identifier.issn | 1949-2553 | |
dc.description | Published | en_GB |
dc.description | Article | en_GB |
dc.identifier.journal | Oncotarget | en_GB |