dc.contributor.author | Alexandrou, AJ | |
dc.contributor.author | Brown, AR | |
dc.contributor.author | Chapman, ML | |
dc.contributor.author | Estacion, M | |
dc.contributor.author | Turner, J | |
dc.contributor.author | Mis, MA | |
dc.contributor.author | Wilbrey, A | |
dc.contributor.author | Payne, EC | |
dc.contributor.author | Gutteridge, A | |
dc.contributor.author | Cox, PJ | |
dc.contributor.author | Doyle, R | |
dc.contributor.author | Printzenhoff, D | |
dc.contributor.author | Lin, Z | |
dc.contributor.author | Marron, BE | |
dc.contributor.author | West, C | |
dc.contributor.author | Swain, NA | |
dc.contributor.author | Storer, RI | |
dc.contributor.author | Stupple, PA | |
dc.contributor.author | Castle, NA | |
dc.contributor.author | Hounshell, JA | |
dc.contributor.author | Rivara, M | |
dc.contributor.author | Randall, A | |
dc.contributor.author | Dib-Hajj, SD | |
dc.contributor.author | Krafte, D | |
dc.contributor.author | Waxman, SG | |
dc.contributor.author | Patel, MK | |
dc.contributor.author | Butt, RP | |
dc.contributor.author | Stevens, EB | |
dc.date.accessioned | 2016-05-05T08:46:35Z | |
dc.date.issued | 2016-04-06 | |
dc.description.abstract | Human genetic studies show that the voltage gated sodium channel 1.7 (Nav1.7) is a key molecular determinant of pain sensation. However, defining the Nav1.7 contribution to nociceptive signalling has been hampered by a lack of selective inhibitors. Here we report two potent and selective arylsulfonamide Nav1.7 inhibitors; PF-05198007 and PF-05089771, which we have used to directly interrogate Nav1.7's role in nociceptor physiology. We report that Nav1.7 is the predominant functional TTX-sensitive Nav in mouse and human nociceptors and contributes to the initiation and the upstroke phase of the nociceptor action potential. Moreover, we confirm a role for Nav1.7 in influencing synaptic transmission in the dorsal horn of the spinal cord as well as peripheral neuropeptide release in the skin. These findings demonstrate multiple contributions of Nav1.7 to nociceptor signalling and shed new light on the relative functional contribution of this channel to peripheral and central noxious signal transmission. | en_GB |
dc.description.sponsorship | The funder provided support in the form of
salaries for authors [AA, AB, MC, JT, MM, AW, EP,
AG, PJC, RD, DP, ZL, BM, CW, NS, RS, PS, NC, DK,
RB, ES], but did not have any additional role in the
study design, data collection and analysis, decision to
publish, or preparation of the manuscript. The specific
roles of these authors are articulated in the ‘author
contributions’ section. | en_GB |
dc.identifier.citation | PLoS One, 2016, Vol. 11 (4): e0152405 | en_GB |
dc.identifier.doi | 10.1371/journal.pone.0152405 | |
dc.identifier.uri | http://hdl.handle.net/10871/21386 | |
dc.language.iso | en | en_GB |
dc.publisher | Public Library of Science | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/27050761 | en_GB |
dc.rights | This is the final version of the article. Available from PLoS via the DOI in this record. | en_GB |
dc.title | Subtype-Selective Small Molecule Inhibitors Reveal a Fundamental Role for Nav1.7 in Nociceptor Electrogenesis, Axonal Conduction and Presynaptic Release. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2016-05-05T08:46:35Z | |
dc.identifier.issn | 1932-6203 | |
exeter.place-of-publication | United States | |
dc.identifier.journal | PLoS One | en_GB |