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dc.contributor.authorWood, ME
dc.contributor.authorChatzianastasiou, A
dc.contributor.authorBibli, S-I
dc.contributor.authorAndreadou, I
dc.contributor.authorEfentakis, P
dc.contributor.authorKaludercic, N
dc.contributor.authorWhiteman, M
dc.contributor.authorDi Lisa, F
dc.contributor.authorDaiber, A
dc.contributor.authorManolopoulos, VG
dc.contributor.authorSzabo, C
dc.contributor.authorPapapetropoulos, A
dc.date.accessioned2016-07-07T06:56:14Z
dc.date.issued2016-08-01
dc.description.abstractHydrogen sulfide (H2S) is a signaling molecule with protective effects in the cardiovascular system. To harness the therapeutic potential of H2S, a number of donors have been developed. The present study compares the cardioprotective actions of representative H2S donors from different classes and studies their mechanisms of action in myocardial injury in vitro and in vivo. Exposure of cardiomyocytes to H2O2 led to significant cytotoxicity, which was inhibited by sodium sulfide (Na2S), thiovaline (TV), GYY4137 [morpholin-4-ium 4 methoxyphenyl(morpholino) phosphinodithioate], and AP39 [(10-oxo-10-(4-(3-thioxo-3H-1,2-dithiol5yl)phenoxy)decyl) triphenylphospho-nium bromide]. Inhibition of nitric oxide (NO) synthesis prevented the cytoprotective effects of Na2S and TV, but not GYY4137 and AP39, against H2O2-induced cardiomyocyte injury. Mice subjected to left anterior descending coronary ligation were protected from ischemia-reperfusion injury by the H2S donors tested. Inhibition of nitric oxide synthase (NOS) in vivo blocked only the beneficial effect of Na2S. Moreover, Na2S, but not AP39, administration enhanced the phosphorylation of endothelial NOS and vasodilator-associated phosphoprotein. Both Na2S and AP39 reduced infarct size in mice lacking cyclophilin-D (CypD), a modulator of the mitochondrial permeability transition pore (PTP). Nevertheless, only AP39 displayed a direct effect on mitochondria by increasing the mitochondrial Ca2+ retention capacity, which is evidence of decreased propensity to undergo permeability transition. We conclude that although all the H2S donors we tested limited infarct size, the pathways involved were not conserved. Na2S had no direct effects on PTP opening, and its action was nitric oxide dependent. In contrast, the cardioprotection exhibited by AP39 could result from a direct inhibitory effect on PTP acting at a site different than CypD.en_GB
dc.identifier.citationvol. 358, no. 3, pp. 431-440en_GB
dc.identifier.doihttp://dx.doi.org/10.1124/jpet.116.235119
dc.identifier.urihttp://hdl.handle.net/10871/22426
dc.language.isoenen_GB
dc.publisherAmerican Society for Pharmacology and Experimental Therapeutics (ASPET)en_GB
dc.relation.urlhttp://jpet.aspetjournals.org/en_GB
dc.rights.embargoreasonUnder indefinite embargo due to publisher policy. The accepted version is freely available from the publisher via the DOI in this record.en_GB
dc.titleCardioprotection by H2S donors: nitric oxide-dependent and -independent mechanismsen_GB
dc.typeArticleen_GB
dc.identifier.issn0022-3565
dc.descriptionArticleen_GB
dc.identifier.eissn1521-0103
dc.identifier.journalJournal of Pharmacology and Experimental Therapeuticsen_GB


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