Automatic thresholding from the gradients of region boundaries
Journal of Microscopy
This is the final version of the article. Available from Wiley via the DOI in this record.
We present an approach for automatic threshold segmentation of greyscale images. The procedure is inspired by a reinterpretation of the strategy observed in human operators when adjusting thresholds manually and interactively by means of ‘slider’ controls. The approach translates into two methods. The first one is suitable for single or multiple global thresholds to be applied globally to images and consists of searching for a threshold value that generates a phase whose boundary coincides with the largest gradients in the original image. The second method is a variation, implemented to operate on the discrete connected components of the thresholded phase (i.e. the binary regions) independently. Consequently, this becomes an adaptive local threshold procedure, which operates relative to regions, rather than to local image subsets as is the case in most local thresholding methods previously published. Adding constraints for specifying certain classes of expected objects in the images can improve the output of the method over the traditional ‘segmenting first, then classify’ approach.
The research reported in this paper was supported by the Engineering and Physical Sciences Research Council (EPSRC), UK through funding under grant EP/M023869/1 ‘Novel contextbased segmentation algorithms for intelligent microscopy’.