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dc.contributor.authorCostello, JL
dc.contributor.authorCastro, , I
dc.contributor.authorHacker, C
dc.contributor.authorSchrader, T
dc.contributor.authorMetz, J
dc.contributor.authorZeuschner, D
dc.contributor.authorAzadi, A
dc.contributor.authorGodinho, L
dc.contributor.authorCostina, V
dc.contributor.authorFindeisen, P
dc.contributor.authorManner, A
dc.contributor.authorIslinger, M
dc.contributor.authorSchrader, M
dc.date.accessioned2017-01-23T12:00:20Z
dc.date.issued2017-01-20
dc.description.abstractPeroxisomes (POs) and the endoplasmic reticulum (ER) cooperate in cellular lipid metabolism and form tight structural associations, which were first observed in ultrastructural studies decades ago. PO–ER associations have been suggested to impact on a diverse number of physiological processes, including lipid metabolism, phospholipid exchange, metabolite transport, signaling, and PO biogenesis. Despite their fundamental importance to cell metabolism, the mechanisms by which regions of the ER become tethered to POs are unknown, in particular in mammalian cells. Here, we identify the PO membrane protein acyl-coenzyme A–binding domain protein 5 (ACBD5) as a binding partner for the resident ER protein vesicle-associated membrane protein-associated protein B (VAPB). We show that ACBD5–VAPB interaction regulates PO–ER associations. Moreover, we demonstrate that loss of PO–ER association perturbs PO membrane expansion and increases PO movement. Our findings reveal the first molecular mechanism for establishing PO–ER associations in mammalian cells and report a new function for ACBD5 in PO–ER tethering.en_GB
dc.description.sponsorshipThis work was supported by grants from the Biotechnology and Biological Sciences Research Council (BB/K006231/1 and BB/ N01541X/1 to M. Schrader). J. Metz and M. Schrader are supported by a Wellcome Trust Institutional Strategic Support Award (WT097835MF and WT105618MA) and L.F. Godinho by a fellowship from Fundação para a Ciência e a Tecnologia, Portugal (SFRH/ BPD/90084/2012). M. Schrader and A.S. Azadi are supported by Marie Curie Initial Training Network action PerFuMe (316723). M. Islinger is supported by MEAMEDMA Anschubförderung, Medical Faculty Mannheim, University of Heidelberg.en_GB
dc.identifier.citationDOI: 10.1083/jcb.201607055en_GB
dc.identifier.doi10.1083/jcb.201607055
dc.identifier.urihttp://hdl.handle.net/10871/25341
dc.language.isoenen_GB
dc.publisherRockefeller University Pressen_GB
dc.rights© 2017 Costello et al. This article is distributed under the terms of an Attribution– Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).en_GB
dc.titleACBD5 and VAPB mediate membrane associations between peroxisomes and the ERen_GB
dc.typeArticleen_GB
dc.date.available2017-01-23T12:00:20Z
dc.identifier.issn0021-9525
dc.descriptionThis is the final version of the article. Available from the publisher via the DOI in this record.en_GB
dc.identifier.journalJournal of Cell Biologyen_GB


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