A label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes
Cama, J; Chimerel, C; Pagliara, S; et al.Javer, A; Keyser, UF
Date: 7 July 2014
Journal
Lab Chip
Publisher
Royal Society of Chemistry
Publisher DOI
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Abstract
With the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes is gaining widespread importance. We present a novel, label-free microfluidic assay that quantifies the permeability coefficient of a broad spectrum fluoroquinolone antibiotic, norfloxacin, across ...
With the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes is gaining widespread importance. We present a novel, label-free microfluidic assay that quantifies the permeability coefficient of a broad spectrum fluoroquinolone antibiotic, norfloxacin, across lipid membranes using the UV autofluorescence of the drug. We use giant lipid vesicles as highly controlled model systems to study the diffusion through lipid membranes. Our technique directly determines the permeability coefficient without requiring the measurement of the partition coefficient of the antibiotic.
Biosciences - old structure
Collections of Former Colleges
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