dc.contributor.author | Munkley, J | |
dc.contributor.author | Oltean, S | |
dc.contributor.author | Vodák, D | |
dc.contributor.author | Wilson, BT | |
dc.contributor.author | Livermore, KE | |
dc.contributor.author | Zhou, Y | |
dc.contributor.author | Star, E | |
dc.contributor.author | Floros, VI | |
dc.contributor.author | Johannessen, B | |
dc.contributor.author | Knight, B | |
dc.contributor.author | McCullagh, P | |
dc.contributor.author | McGrath, J | |
dc.contributor.author | Crundwell, M | |
dc.contributor.author | Skotheim, RI | |
dc.contributor.author | Robson, CN | |
dc.contributor.author | Leung, HY | |
dc.contributor.author | Harries, LW | |
dc.contributor.author | Rajan, P | |
dc.contributor.author | Mills, IG | |
dc.contributor.author | Elliott, DJ | |
dc.date.accessioned | 2017-02-13T14:44:17Z | |
dc.date.issued | 2015-10-07 | |
dc.description.abstract | Patterns of glycosylation are important in cancer, but the molecular mechanisms that drive changes are often poorly understood. The androgen receptor drives prostate cancer (PCa) development and progression to lethal metastatic castration-resistant disease. Here we used RNA-Seq coupled with bioinformatic analyses of androgen-receptor (AR) binding sites and clinical PCa expression array data to identify ST6GalNAc1 as a direct and rapidly activated target gene of the AR in PCa cells. ST6GalNAc1 encodes a sialytransferase that catalyses formation of the cancer-associated sialyl-Tn antigen (sTn), which we find is also induced by androgen exposure. Androgens induce expression of a novel splice variant of the ST6GalNAc1 protein in PCa cells. This splice variant encodes a shorter protein isoform that is still fully functional as a sialyltransferase and able to induce expression of the sTn-antigen. Surprisingly, given its high expression in tumours, stable expression of ST6GalNAc1 in PCa cells reduced formation of stable tumours in mice, reduced cell adhesion and induced a switch towards a more mesenchymal-like cell phenotype in vitro. ST6GalNAc1 has a dynamic expression pattern in clinical datasets, beingsignificantly up-regulated in primary prostate carcinoma but relatively down-regulated in established metastatic tissue. ST6GalNAc1 is frequently upregulated concurrently with another important glycosylation enzyme GCNT1 previously associated with prostate cancer progression and implicated in Sialyl Lewis X antigen synthesis. Together our data establishes an androgen-dependent mechanism for sTn antigen expression in PCa, and are consistent with a general role for the androgen receptor in driving important coordinate changes to the glycoproteome during PCa progression. | en_GB |
dc.description.sponsorship | The authors would like to thank Mr Robert Jackson and Mr Ben Lee for technical assistance, Dr Stuart Williamson for kindly providing us with the prostate tissue lysates used in this study and Exeter NIHR Clinical Research Facility for providing patient RNA. This work was funded by Prostate Cancer UK (PG12-34), The J. G. W Patterson Foundation, The Wellcome Trust (grant numbers WT080368MA and WT089225/Z/09/Z) and BBSRC (grant BB/1006923/1 and BB/J007293/1). I.G.M. is supported in Oslo by funding the Norwegian Research Council, Helse Sor-Ost and the University of Oslo through the Centre for Molecular Medicine (Norway), which is the part of the Nordic EMBL (European Molecular Biology Laboratory) partnership and also supported by Oslo University Hospitals. I.G.M. is also supported by the Norwegian Cancer Society. I.G.M. holds a visiting scientist position with Cancer Research UK through the Cambridge Research Institute and a Senior Visiting Research Fellowship with Cambridge University through the Department of Oncology. I.G.M is supported in Belfast by the Belfast-Manchester Movember Centre of Excellence (CE013_2-004), funded in partnership with Prostate Cancer UK. | en_GB |
dc.identifier.citation | Vol. 6, pp. 34358 - 34374 | en_GB |
dc.identifier.doi | 10.18632/oncotarget.6024 | |
dc.identifier.uri | http://hdl.handle.net/10871/25794 | |
dc.language.iso | en | en_GB |
dc.publisher | Impact Journals | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/26452038 | en_GB |
dc.rights | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_GB |
dc.subject | ST6GalNAc1 | en_GB |
dc.subject | Sialyl-Tn (sTn) | en_GB |
dc.subject | androgens | en_GB |
dc.subject | glycosylation | en_GB |
dc.subject | prostate cancer | en_GB |
dc.subject | Animals | en_GB |
dc.subject | Antigens, CD | en_GB |
dc.subject | Antigens, Tumor-Associated, Carbohydrate | en_GB |
dc.subject | Cell Adhesion | en_GB |
dc.subject | Chromatin Immunoprecipitation | en_GB |
dc.subject | Gene Expression Regulation, Neoplastic | en_GB |
dc.subject | Gene Knockdown Techniques | en_GB |
dc.subject | Heterografts | en_GB |
dc.subject | Humans | en_GB |
dc.subject | Male | en_GB |
dc.subject | Mice | en_GB |
dc.subject | Mice, Nude | en_GB |
dc.subject | Prostatic Neoplasms | en_GB |
dc.subject | RNA, Small Interfering | en_GB |
dc.subject | Receptors, Androgen | en_GB |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en_GB |
dc.subject | Sialyltransferases | en_GB |
dc.subject | Transfection | en_GB |
dc.title | The androgen receptor controls expression of the cancer-associated sTn antigen and cell adhesion through induction of ST6GalNAc1 in prostate cancer. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2017-02-13T14:44:17Z | |
exeter.place-of-publication | United States | en_GB |
dc.description | This is the final version of the article. Available from Impact Journals via the DOI in this record. | en_GB |
dc.identifier.eissn | 1949-2553 | |
dc.identifier.journal | Oncotarget | en_GB |