Coccolithophores: functional biodiversity, enzymes and bioprospecting
© 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Emiliania huxleyi is a single celled, marine phytoplankton with global distribution. As a key species for global biogeochemical cycling, a variety of strains have been amassed in various culture collections. Using a library consisting of 52 strains of E. huxleyi and an 'in house' enzyme screening program, we have assessed the functional biodiversity within this species of fundamental importance to global biogeochemical cycling, whilst at the same time determining their potential for exploitation in biocatalytic applications. Here, we describe the screening of E. huxleyi strains, as well as a coccolithovirus infected strain, for commercially relevant biocatalytic enzymes such as acid/alkali phosphodiesterase, acid/alkali phosphomonoesterase, EC1.1.1-type dehydrogenase, EC1.3.1-type dehydrogenase and carboxylesterase.
The authors would like to acknowledge Andrew Willets for establishing the enzyme screening program at PML Applications Ltd as part of the NERC follow on fund NE/F014406/1 awarded to STA, also supported by i-GPeninsula, a Public Sector Research Exploitation project funded by the Department of Business, Innovation, and Skills (HM Government, UK). MJA, PJS and STA are supported by NERC’s Oceans 2025 program. ER and CW are supported by BBSRC Industrial CASE studentships, sponsored by PML Applications Ltd. MJA, IP and CW were supported by funds from the Interreg program, Marinexus. JL and JN are supported by the BBSRC. Strains from the RCC were obtained through the ASSEMBLE program (FP7-227799).
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Vol. 9 (4), pp. 586 - 602
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