Labeling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago maydis
Guimarães, S; Kilaru, S; Schrader, M; et al.Schuster, M
Date: 14 April 2017
Article
Journal
Methods in Molecular Biology
Publisher
Humana Press (Springer Imprint)
Publisher DOI
Abstract
The basidiomycete fungus Ustilago maydis has emerged as a powerful model organism
to study fundamental biological processes. U. maydis shares many important features
with human cells but provides the technical advantages of yeast. Recently, U. maydis
has also been used to investigate fundamental processes in peroxisome biology. Here, ...
The basidiomycete fungus Ustilago maydis has emerged as a powerful model organism
to study fundamental biological processes. U. maydis shares many important features
with human cells but provides the technical advantages of yeast. Recently, U. maydis
has also been used to investigate fundamental processes in peroxisome biology. Here, we present an efficient yeast recombination-based cloning method to construct and express fluorescent fusion proteins (or conditional mutant protein alleles) which target peroxisomes in the fungus U. maydis. In vivo analysis is pivotal for understanding the underlying mechanisms of organelle motility. We focus on the in vivo labelling of peroxisomes in U. maydis and present approaches to analyze peroxisomal motility.
Biosciences - old structure
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