Peroxisomal ACBD4 interacts with VAPB and promotes ER-peroxisome associations
Taylor & Francis
© 2017 The Author(s). Published with license by Taylor & Francis© Joseph L. Costello, Inês G. Castro, Tina A. Schrader, Markus Islinger, and Michael Schrader This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
Cooperation between cellular organelles such as mitochondria, peroxisomes and the ER is essential for a variety of important and diverse metabolic processes. Effective communication and metabolite exchange requires physical linkages between the organelles, predominantly in the form of organelle contact sites. At such contact sites organelle membranes are brought into close proximity by the action of molecular tethers, which often consist of specific protein pairs anchored in the membrane of the opposing organelles. Currently numerous tethering components have been identified which link the ER with multiple other organelles but knowledge of the factors linking the ER with peroxisomes is limited. Peroxisome-ER interplay is important because it is required for the biosynthesis of unsaturated fatty acids, ether-phospholipids and sterols with defects in these functions leading to severe diseases. Here we characterise acyl-CoA binding domain protein 4 (ACBD4) as a tail-anchored peroxisomal membrane protein which interacts with the ER protein, vesicle-associated membrane protein-associated protein–B (VAPB) to promote peroxisome-ER associations.
We thank all colleagues who provided plasmids and antibodies, and T Levine for sharing data. This work was supported by BBSRC (BB/K006231/1, BB/N01541X/1). MS is supported by the Marie Curie Initial Training Network action PerFuMe (316723). The authors declare no competing financial interests.
This is the author accepted manuscript. The final version is available from Taylor & Francis via the DOI in this record.
Published online: 02 May 2017