Metagenomic analysis of silage
Tennant, RK; Sambles, CM; Diffey, GE; et al.Moore, KA; Love, J
Date: 13 January 2017
Journal
Journal of Visualized Experiments
Publisher
MyJoVE Corp.
Publisher DOI
Abstract
Metagenomics is defined as the direct analysis of deoxyribonucleic acid (DNA) purified from environmental samples and enables taxonomic
identification of the microbial communities present within them. Two main metagenomic approaches exist; sequencing the 16S rRNA gene
coding region, which exhibits sufficient variation between taxa ...
Metagenomics is defined as the direct analysis of deoxyribonucleic acid (DNA) purified from environmental samples and enables taxonomic
identification of the microbial communities present within them. Two main metagenomic approaches exist; sequencing the 16S rRNA gene
coding region, which exhibits sufficient variation between taxa for identification, and shotgun sequencing, in which genomes of the organisms
that are present in the sample are analyzed and ascribed to "operational taxonomic units"; species, genera or families depending on the extent of
sequencing coverage.
In this study, shotgun sequencing was used to analyze the microbial community present in cattle silage and, coupled with a range of
bioinformatics tools to quality check and filter the DNA sequence reads, perform taxonomic classification of the microbial populations present
within the sampled silage, and achieve functional annotation of the sequences. These methods were employed to identify potentially harmful
bacteria that existed within the silage, an indication of silage spoilage. If spoiled silage is not remediated, then upon ingestion it could be
potentially fatal to the livestock.
Biosciences - old structure
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