CD21(int) CD23(+) follicular B cells express antigen-specific secretory IgM mRNA as primary and memory responses
dc.contributor.author | Meng, Q-H | |
dc.contributor.author | White, HN | |
dc.date.accessioned | 2017-06-09T12:15:01Z | |
dc.date.issued | 2017-03-16 | |
dc.description.abstract | CD21(int) CD23(+) IgM(+) mouse follicular B cells comprise the bulk of the mature B-cell compartment, but it is not known whether these cells contribute to the humoral antibody response. We show using a direct RT-PCR method for antigen-specific VH, that FACS-sorted mouse CD21(int) CD23(+) B cells express specific secretory IgM VH transcripts in response to immunization and also exhibit a memory response. The secretory IgM expressed is distinct from the IgG expressed by cells of this phenotype, which we also analyse here, having a distinct broader distribution of CDR-H3 sequences and zero or low levels of somatic mutation in the region analysed. These results imply that cells of the CD21(int) CD23(+) phenotype have distinct IgM(+) and IgG(+) populations that contribute directly to the humoral antibody and memory responses by expressing antigen-specific secretory immunoglobulin. We also argue that the more diverse CDR-H3 sequences expressed by antigen-experienced IgM(+) CD21(int) CD23(+) follicular B cells would place them at the bottom of a recently hypothesized memory B-cell hierarchy. | en_GB |
dc.description.sponsorship | This work was funded by Wellcome Trust grant 062578. | en_GB |
dc.identifier.citation | Vol. 151(2), pp. 211 - 218 | en_GB |
dc.identifier.doi | 10.1111/imm.12724 | |
dc.identifier.uri | http://hdl.handle.net/10871/27910 | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley for British Society for Immunology | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/28190261 | en_GB |
dc.rights | © 2017 The Authors. Immunology Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | B cells | en_GB |
dc.subject | follicular | en_GB |
dc.subject | memory | en_GB |
dc.subject | repertoire | en_GB |
dc.title | CD21(int) CD23(+) follicular B cells express antigen-specific secretory IgM mRNA as primary and memory responses | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2017-06-09T12:15:01Z | |
exeter.place-of-publication | England | en_GB |
dc.description | This is the final version of the article. Available from Wiley via the DOI in this record. | en_GB |
dc.identifier.journal | Immunology | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ |
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Except where otherwise noted, this item's licence is described as © 2017 The Authors. Immunology Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.