dc.contributor.author | Sambles, C | |
dc.contributor.author | Moore, K | |
dc.contributor.author | Lux, TM | |
dc.contributor.author | Jones, K | |
dc.contributor.author | Littlejohn, GR | |
dc.contributor.author | Gouveia, JD | |
dc.contributor.author | Aves, SJ | |
dc.contributor.author | Studholme, DJ | |
dc.contributor.author | Lee, R | |
dc.contributor.author | Love, J | |
dc.date.accessioned | 2017-07-03T10:18:39Z | |
dc.date.issued | 2017-06-28 | |
dc.description.abstract | Microalgae are widely viewed as a promising and sustainable source of renewable
chemicals and biofuels. Botryococcus braunii synthesizes and secretes significant
amounts of long-chain (C30-C40) hydrocarbons that can be subsequently converted
into gasoline, diesel, and aviation fuel. B. braunii cultures are not axenic and the effects
of co-cultured microorganisms on B. braunii growth and hydrocarbon yield are important,
but sometimes contradictory. To understand the composition of the B. braunii
microbial consortium, we used high throughput Illumina sequencing of metagenomic
DNA to profile the microbiota within a well established, stable B. braunii culture and
characterized the demographic changes in the microcosm following modification to
the culture conditions. DNA sequences attributed to B. braunii were present in equal
quantities in all treatments, whereas sequences assigned to the associated microbial
community were dramatically altered. Bacterial species least affected by treatments,
and more robustly associated with the algal cells, included members of Rhizobiales,
comprising Bradyrhizobium and Methylobacterium, and representatives of Dyadobacter,
Achromobacter and Asticcacaulis. The presence of bacterial species identified by
metagenomics was confirmed by additional 16S rDNA analysis of bacterial isolates.
Our study demonstrates the advantages of high throughput sequencing and robust
metagenomic analyses to define microcosms and further our understanding of microbial
ecology. | en_GB |
dc.description.sponsorship | Shell Research Limited; Biotechnology and
Biological Sciences Research Council, Grant/
Award Number: BB/K003240/2 and BB/
K003240/1; Medical Research Council Clinical
Infrastructure award, Grant/Award Number:
MR/M008924/1; Wellcome Trust Institutional
Strategic Support Fund, Grant/Award Number:
WT097835MF; Wellcome Trust Multi User
Equipment Award, Grant/Award Number:
WT101650MA | en_GB |
dc.identifier.citation | Vol. 6 (4), article e00482 | en_GB |
dc.identifier.doi | 10.1002/mbo3.482 | |
dc.identifier.uri | http://hdl.handle.net/10871/28281 | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley | en_GB |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium,
provided the original work is properly cited.
© 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. | en_GB |
dc.subject | biofuel | en_GB |
dc.subject | Botryococcus braunii | en_GB |
dc.subject | consortium | en_GB |
dc.subject | metagenomics | en_GB |
dc.subject | microcosm | en_GB |
dc.title | Metagenomic analysis of the complex microbial consortium associated with cultures of the oil-rich alga Botryococcus braunii | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2017-07-03T10:18:39Z | |
dc.identifier.issn | 2045-8827 | |
dc.description | This is the final version of the article. Available from the publisher via the DOI in this record. | en_GB |
dc.identifier.journal | MicrobiologyOpen | en_GB |
refterms.dateFOA | 2023-08-21T15:10:11Z | |