Modelling microbial transport in simulated low-grade heap bioleaching systems: The hydrodynamic dispersion model
Chemical Engineering Science
Elsevier for Pergamon
© 2017 Published by Elsevier Ltd.
Reason for embargo
The hydrodynamic model was developed to describe microbial growth kinetics within heap bioleaching systems. Microbial partitioning between the bulk flowing pregnant leach solution (PLS) and ore-associated phases that exist within the low-grade chalcopyrite ore bed, as a function of microbial transport between these identified phases, was investigated. Microbial transport between the bulk flowing PLS and ore-associated phases was postulated to be driven by the microbial concentration gradient between the phases, with advection and dispersion forces facilitating microbial colonisation of, and transport through, the ore bed. The population balance model (PBM) was incorporated into the hydrodynamic model to estimate mineral dissolution rates as a function of available surface area appropriately. Temporal and spatial variations in microbial concentration in the PLS and ore-associated phases are presented together with model predictions for overall ferrous and ferric iron concentrations, which account for iron concentrations in the bulk flowing PLS and that in the vicinity of the mineral surface. The model predictions for PLS and ore-associated microbial concentrations are validated with experimental data, demonstrating the improvement of this model over the previously presented ‘biomass model’. Based on Michaelis-Menten type kinetics, model-predicted true maximum specific growth rates for Acidithiobacillus ferrooxidans in the PLS and ore-associated phases were found to be 0.0004 and 0.019 h −1 , respectively. Estimated microbial attachment and detachment rates suggest that microbial growth is more prolific in the ore-associated phases with subsequent transport to the bulk flowing PLS. Sensitivity analysis of the hydrodynamic transport model to changes in the advection mass transfer coefficient, dispersion coefficient and inoculum size are discussed. For the current reactor configuration, increasing the irrigation rate from 2 to 2.5 L m −2 h −1 , i.e. increasing the advection mass transfer rate, resulted in a significant decrease in microbial retention within the ore bed.
The financial assistance of the Department of Science and Technology (DST) and the National Research Foundation (NRF) of South Africa, through the South African Research Chairs Initiative (SARChI UID64778) is hereby acknowledged. Opinions expressed and conclusions arrived, are those of the author and are not necessarily to be attributed to the NRF.
This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record.
Vol. 172, pp. 545 - 558
Showing items related by title, author, creator and subject.
Microbial metabolism of alkylbenzene sulphonates. Bacterial metabolism of undecylbenzene-p-sulphonate and dodecylbenzene-p-sulphonate Willetts, A. J.; Cain, R. B. (1972-09)1. A study was made of the biodegradation of alkylbenzene sulphonate homologues, one of the major components of commercially marketed detergents. A Bacillus species was elected for growth on alkylbenzene sulphonate homologues ...
A new and diverse plastid-bearing microbial eukaryote and its position on the eukaryotic tree of life. Harrison, James William (University of ExeterBiosciences, 2010-09-30)Using group-specific environmental clone libraries to target a section of the plastid 16S SSU rDNA gene we have identified a novel plastid-bearing eukaryotic lineage and have named this group “rappemonads” in anticipation ...
Bapteste, Eric; Dupré, John (Springer Verlag, 2013-03)Standard microbial evolutionary ontology is organized according to a nested hierarchy of entities at various levels of biological organization. It typically detects and defines these entities in relation to the most ...