Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography.
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We employed electron cryo-tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation-arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ribosome recruitment and stabilization. Ribosomes are bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport. Our work provides unique insights into the spatial organization of cytosolic ribosomes on mitochondria.
This work was supported by the Max Planck Society, University of Exeter, Foundation for Polish Science—Welcome Programme co‐financed by the EU within the European Regional Development Fund and National Science Centre, Poland (NCN), grant DEC‐2013/11/B/NZ3/00974. P.B. was supported by NCN grant DEC‐2013/11/D/NZ1/02294.
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Vol. 18, No. 9, pp 1473 - 1671
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