The dynamic architecture of the metabolic switch in Streptomyces coelicolor
dc.contributor.author | Nieselt, K | |
dc.contributor.author | Battke, F | |
dc.contributor.author | Herbig, A | |
dc.contributor.author | Bruheim, P | |
dc.contributor.author | Wentzel, A | |
dc.contributor.author | Jakobsen, ØM | |
dc.contributor.author | Sletta, H | |
dc.contributor.author | Alam, MT | |
dc.contributor.author | Merlo, ME | |
dc.contributor.author | Moore, J | |
dc.contributor.author | Omara, WAM | |
dc.contributor.author | Morrissey, ER | |
dc.contributor.author | Juarez-Hermosillo, MA | |
dc.contributor.author | Rodríguez-García, A | |
dc.contributor.author | Nentwich, M | |
dc.contributor.author | Thomas, L | |
dc.contributor.author | Iqbal, M | |
dc.contributor.author | Legaie, R | |
dc.contributor.author | Gaze, WH | |
dc.contributor.author | Challis, GL | |
dc.contributor.author | Jansen, RC | |
dc.contributor.author | Dijkhuizen, L | |
dc.contributor.author | Rand, DA | |
dc.contributor.author | Wild, DL | |
dc.contributor.author | Bonin, M | |
dc.contributor.author | Reuther, J | |
dc.contributor.author | Wohlleben, W | |
dc.contributor.author | Smith, MCM | |
dc.contributor.author | Burroughs, NJ | |
dc.contributor.author | Martín, JF | |
dc.contributor.author | Hodgson, DA | |
dc.contributor.author | Takano, E | |
dc.contributor.author | Breitling, R | |
dc.contributor.author | Ellingsen, TE | |
dc.contributor.author | Wellington, EMH | |
dc.date.accessioned | 2017-09-26T10:58:16Z | |
dc.date.issued | 2010-01-06 | |
dc.description.abstract | BACKGROUND: During the lifetime of a fermenter culture, the soil bacterium S. coelicolor undergoes a major metabolic switch from exponential growth to antibiotic production. We have studied gene expression patterns during this switch, using a specifically designed Affymetrix genechip and a high-resolution time-series of fermenter-grown samples. RESULTS: Surprisingly, we find that the metabolic switch actually consists of multiple finely orchestrated switching events. Strongly coherent clusters of genes show drastic changes in gene expression already many hours before the classically defined transition phase where the switch from primary to secondary metabolism was expected. The main switch in gene expression takes only 2 hours, and changes in antibiotic biosynthesis genes are delayed relative to the metabolic rearrangements. Furthermore, global variation in morphogenesis genes indicates an involvement of cell differentiation pathways in the decision phase leading up to the commitment to antibiotic biosynthesis. CONCLUSIONS: Our study provides the first detailed insights into the complex sequence of early regulatory events during and preceding the major metabolic switch in S. coelicolor, which will form the starting point for future attempts at engineering antibiotic production in a biotechnological setting. | en_GB |
dc.description.sponsorship | This project was supported by grants of the ERA-NET SySMO Project [GEN2006-27745-E/SYS]: (P-UK-01-11-3i) and the Research Council of Norway [project no. 181840/I30]. | en_GB |
dc.identifier.citation | Vol. 11, article 10 | en_GB |
dc.identifier.doi | 10.1186/1471-2164-11-10 | |
dc.identifier.uri | http://hdl.handle.net/10871/29539 | |
dc.language.iso | en | en_GB |
dc.publisher | BioMed Central | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/20053288 | en_GB |
dc.rights | © Nieselt et al; licensee BioMed Central Ltd. 2010 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | Anti-Bacterial Agents | en_GB |
dc.subject | Cluster Analysis | en_GB |
dc.subject | Fermentation | en_GB |
dc.subject | Gene Expression Profiling | en_GB |
dc.subject | Gene Expression Regulation, Bacterial | en_GB |
dc.subject | Genes, Bacterial | en_GB |
dc.subject | Multigene Family | en_GB |
dc.subject | RNA, Bacterial | en_GB |
dc.subject | Streptomyces coelicolor | en_GB |
dc.title | The dynamic architecture of the metabolic switch in Streptomyces coelicolor | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2017-09-26T10:58:16Z | |
exeter.place-of-publication | England | en_GB |
dc.description | This is the final version of the article. Available from BioMed Central via the DOI in this record. | en_GB |
dc.identifier.journal | BMC Genomics | en_GB |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0 |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © Nieselt et al; licensee BioMed Central Ltd. 2010 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.