Barcoding in trypanosomes.
Cambridge University Press (CUP)
COPYRIGHT: © Cambridge University Press 2017. This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Trypanosomes (genus Trypanosoma) are parasites of humans, and wild and domestic mammals, in which they cause several economically and socially important diseases, including sleeping sickness in Africa and Chagas disease in the Americas. Despite the development of numerous molecular diagnostics and increasing awareness of the importance of these neglected parasites, there is currently no universal genetic barcoding marker available for trypanosomes. In this review we provide an overview of the methods used for trypanosome detection and identification, discuss the potential application of different barcoding techniques and examine the requirements of the 'ideal' trypanosome genetic barcode. In addition, we explore potential alternative genetic markers for barcoding Trypanosoma species, including an analysis of phylogenetically informative nucleotide changes along the length of the 18S rRNA gene.
R. H. is funded by a UK Biotechnology and Biological Sciences Research Council PhD studentship (grant No. BB/M009122/1) held by Professor Wendy Gibson (Bristol) and J. R. S., as part of the South West Doctoral Training Partnership (SWBio DTP).
This is the final version of the article. Available from Cambridge University Press via the DOI in this record.
Published online: 23 November 2017
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