dc.contributor.author | Fortunato, TM | |
dc.contributor.author | Beltrami, C | |
dc.contributor.author | Emanueli, C | |
dc.contributor.author | De Bank, PA | |
dc.contributor.author | Pula, G | |
dc.date.accessioned | 2018-01-10T09:06:24Z | |
dc.date.issued | 2016-05-04 | |
dc.description.abstract | Revascularisation is a key step for tissue regeneration and complete organ engineering. We describe the generation of human platelet lysate gel (hPLG), an extracellular matrix preparation from human platelets able to support the proliferation of endothelial colony forming cells (ECFCs) in 2D cultures and the formation of a complete microvascular network in vitro in 3D cultures. Existing extracellular matrix preparations require addition of high concentrations of recombinant growth factors and allow only limited formation of capillary-like structures. Additional advantages of our approach over existing extracellular matrices are the absence of any animal product in the composition hPLG and the possibility of obtaining hPLG from patients to generate homologous scaffolds for re-implantation. This discovery has the potential to accelerate the development of regenerative medicine applications based on implantation of microvascular networks expanded ex vivo or the generation of fully vascularised organs. | en_GB |
dc.description.sponsorship | This work was possible thanks to funding from the BBSRC (BB/J002690/1). | en_GB |
dc.identifier.citation | Vol. 6, article 25326 | en_GB |
dc.identifier.doi | 10.1038/srep25326 | |
dc.identifier.uri | http://hdl.handle.net/10871/30885 | |
dc.language.iso | en | en_GB |
dc.publisher | Springer Nature | en_GB |
dc.rights | Open access. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en_GB |
dc.title | Platelet lysate gel and endothelial progenitors stimulate microvascular network formation in vitro: tissue engineering implications | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2018-01-10T09:06:24Z | |
dc.description | This is the final version of the article. Available from Springer Nature via the DOI in this record. | en_GB |
dc.identifier.journal | Scientific Reports | en_GB |