dc.contributor.author | Jeffery, N | |
dc.contributor.author | Richardson, S | |
dc.contributor.author | Beall, C | |
dc.contributor.author | Harries, LW | |
dc.date.accessioned | 2018-01-11T13:51:08Z | |
dc.date.issued | 2017-10-31 | |
dc.description.abstract | Interaction between islet cell subtypes and the extracellular matrix influences beta-cell function in mammals. The tissue architecture of rodent islets is very different to that of human islets; cell-to-cell communication and interaction with the extracellular matrix may vary between species. In this work, we have compared the responses of the human EndoC-βH1 cell line to non-human and human-derived growth matrices in terms of growth morphology, gene expression and glucose-stimulated insulin secretion (GSIS). EndoC-βH1 cells demonstrated a greater tendency to form cell clusters when cultured in a human microenvironment and exhibited reduced alpha cell markers at the mRNA level; mean expression difference - 0.23 and - 0.51; p = 0.009 and 0.002 for the Aristaless-related homeobox (ARX) and Glucagon (GCG) genes respectively. No differences were noted in the protein expression of mature beta cell markers such as Pdx1 and NeuroD1 were noted in EndoC-βH1 cells grown in a human microenvironment but cells were however more sensitive to glucose (4.3-fold increase in insulin secretion following glucose challenge compared with a 1.9-fold increase in cells grown in a non-human microenvironment; p = 0.0003). Our data suggests that the tissue origin of the cellular microenvironment has effects on the function of EndoC-βH1 cells in vitro, and the use of a more human-like culture microenvironment may bring benefits in terms of increased physiological relevance. | en_GB |
dc.description.sponsorship | We would like to gratefully acknowledge funding from Animal Free Research. | en_GB |
dc.identifier.citation | Vol. 361 (2), pp. 284 - 291 | en_GB |
dc.identifier.doi | 10.1016/j.yexcr.2017.10.028 | |
dc.identifier.uri | http://hdl.handle.net/10871/30908 | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/29107069 | en_GB |
dc.rights.embargoreason | Publisher policy | en_GB |
dc.rights | © 2017 Elsevier Inc. All rights reserved. | en_GB |
dc.subject | Beta-cells | en_GB |
dc.subject | Diabetes | en_GB |
dc.subject | Microenvironment | en_GB |
dc.subject | Animals | en_GB |
dc.subject | Basic Helix-Loop-Helix Transcription Factors | en_GB |
dc.subject | Cell Communication | en_GB |
dc.subject | Cell Line | en_GB |
dc.subject | Cellular Microenvironment | en_GB |
dc.subject | Extracellular Matrix | en_GB |
dc.subject | Gene Expression Regulation | en_GB |
dc.subject | Glucagon | en_GB |
dc.subject | Glucose | en_GB |
dc.subject | Homeodomain Proteins | en_GB |
dc.subject | Humans | en_GB |
dc.subject | Insulin | en_GB |
dc.subject | Insulin-Secreting Cells | en_GB |
dc.subject | Mice | en_GB |
dc.subject | Signal Transduction | en_GB |
dc.subject | Species Specificity | en_GB |
dc.subject | Trans-Activators | en_GB |
dc.subject | Transcription Factors | en_GB |
dc.title | The species origin of the cellular microenvironment influences markers of beta cell fate and function in EndoC-βH1 cells | en_GB |
dc.type | Article | en_GB |
exeter.place-of-publication | United States | en_GB |
dc.description | This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record. | en_GB |
dc.identifier.journal | Experimental Cell Research | en_GB |