dc.contributor.author | Tsibidis, GD | |
dc.contributor.author | Burroughs, NJ | |
dc.contributor.author | Gaze, W | |
dc.contributor.author | Wellington, EMH | |
dc.date.accessioned | 2018-02-22T08:31:16Z | |
dc.date.issued | 2011-12 | |
dc.description.abstract | Interaction between bacteria and protozoa is an increasing area of interest, however there are a few systems that allow extensive observation of the interactions. A semi-automated approach is proposed to analyse a large amount of experimental data and avoid a time demanding manual object classification. We examined a surface system consisting of non nutrient agar with a uniform bacterial lawn that extended over the agar surface, and a spatially localised central population of amoebae. Location and identification of protozoa and quantification of bacteria population are performed by the employment of image analysis techniques in a series of spatial images. The quantitative tools are based on intensity thresholding, or on probabilistic models. To accelerate organism identification, correct classification errors and attain quantitative details of all objects a custom written Graphical User Interfaces has also been developed. | en_GB |
dc.identifier.citation | Vol. 42, pp. 911 - 920 | en_GB |
dc.identifier.doi | 10.1016/j.micron.2011.06.010 | |
dc.identifier.uri | http://hdl.handle.net/10871/31615 | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/21775158 | en_GB |
dc.subject | Acanthamoeba | en_GB |
dc.subject | Automation | en_GB |
dc.subject | Image Processing, Computer-Assisted | en_GB |
dc.subject | Microbial Interactions | en_GB |
dc.subject | Microbiological Techniques | en_GB |
dc.subject | Salmonella typhimurium | en_GB |
dc.subject | Time-Lapse Imaging | en_GB |
dc.title | Semi-automated Acanthamoeba polyphaga detection and computation of Salmonella typhimurium concentration in spatio-temporal images. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2018-02-22T08:31:16Z | |
exeter.place-of-publication | England | en_GB |
dc.description | This is the author accepted manuscript. The final version is available from the publisher via the DOI in this record. | en_GB |
dc.identifier.journal | Micron | en_GB |