A single fungal MAP kinase controls plant cell-to-cell invasion by the rice blast fungus
Oliveira Garcia, E
American Association for the Advancement of Science
Blast disease destroys up to 30% of the rice crop annually and threatens global food security. The blast fungusMagnaporthe oryzaeinvades plant tissue with hyphae that proliferate and grow from cell to cell, often through pit fields, where plasmodesmata cluster. We showed that chemical genetic inhibition of a single fungal mitogen-activated protein (MAP) kinase, Pmk1, preventsM. oryzaefrom infecting adjacent plant cells, leaving the fungus trapped within a single plant cell. Pmk1 regulates expression of secreted fungal effector proteins implicated in suppression of host immune defenses, preventing reactive oxygen species generation and excessive callose deposition at plasmodesmata. Furthermore, Pmk1 controls the hyphal constriction required for fungal growth from one rice cell to the neighboring cell, enabling host tissue colonization and blast disease.
This work was funded by a Halpin Scholarship for rice blast research to W.S., a European Research Council (ERC) Advanced Investigator Award to N.J.T. under the European Union’s Seventh Framework Programme (FP7/2007-2013) and ERC grant no. 294702 GENBLAST, and Agriculture and Food Research Initiative Competitive grant no. 2012-67013-19291 to B.V. from the U.S. Department of Agriculture National Institute of Food and Agriculture. This is contribution number 18-174-J from the Kansas Agricultural Experiment Station.
This is the author accepted manuscript.
The final version is available from the publisher via the DOI in this record.
Vol. 359, pp. 1399 - 1403
Place of publication