dc.contributor.author | Isner, J-C | |
dc.contributor.author | Xu, Z | |
dc.contributor.author | Costa, JM | |
dc.contributor.author | Monnet, F | |
dc.contributor.author | Batstone, T | |
dc.contributor.author | Ou, X | |
dc.contributor.author | Deeks, MJ | |
dc.contributor.author | Genty, B | |
dc.contributor.author | Jiang, K | |
dc.contributor.author | Hetherington, AM | |
dc.date.accessioned | 2018-05-14T10:40:22Z | |
dc.date.issued | 2017-08 | |
dc.description.abstract | Stomata respond to darkness by closing to prevent excessive water loss during the night. Although the reorganisation of actin filaments during stomatal closure is documented, the underlying mechanisms responsible for dark-induced cytoskeletal arrangement remain largely unknown. We used genetic, physiological and cell biological approaches to show that reorganisation of the actin cytoskeleton is required for dark-induced stomatal closure. The opal5 mutant does not close in response to darkness but exhibits wild-type (WT) behaviour when exposed to abscisic acid (ABA) or CaCl2 . The mutation was mapped to At5g18410, encoding the PIR/SRA1/KLK subunit of the ArabidopsisSCAR/WAVE complex. Stomata of an independent allele of the PIR gene (Atpir-1) showed reduced sensitivity to darkness and F1 progenies of the cross between opal5 and Atpir-1 displayed distorted leaf trichomes, suggesting that the two mutants are allelic. Darkness induced changes in the extent of actin filament bundling in WT. These were abolished in opal5. Disruption of filamentous actin using latrunculin B or cytochalasin D restored wild-type stomatal sensitivity to darkness in opal5. Our findings suggest that the stomatal response to darkness is mediated by reorganisation of guard cell actin filaments, a process that is finely tuned by the conserved SCAR/WAVE-Arp2/3 actin regulatory module. | en_GB |
dc.description.sponsorship | This
work was supported by grants from the BBSRC (BB/
N001168/1; BB/J002364/1; BBF001177/1), The Gatsby Charitable
Foundation and the Leverhulme Trust to A.M.H. and
grants from the National Natural Science Foundation of China
(nos. 31300213 and 31670408 to K.J.). This work was also
supported by the Centre National de la Recherche Scientifique
and the Commissariat a l’Energie Atomique et aux Energies
Alternatives (for the IR camera) and the European Union
Marie Curie FP5 Research Training Network (program no.
STRESSIMAGING HNRT-CT-2002-00254 to J.M.C. and
B.G.). J.M.C. was in addition supported by a scholarship of
the Fundac ~ao para a Ci^encia e Tecnologia, Portugal (grant no.
SFRH/BPD/34429/2006). | en_GB |
dc.identifier.citation | Vol. 215, pp. 1059 - 1067 | en_GB |
dc.identifier.doi | 10.1111/nph.14655 | |
dc.identifier.uri | http://hdl.handle.net/10871/32825 | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/28636198 | en_GB |
dc.rights | ©2017 The Authors. New Phytologist © 2017 New Phytologist Trust
This is an open access article under the terms of the Creative Commons Attribution License, which permits use,
distribution and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | Arp2/3 complex | en_GB |
dc.subject | SCAR/WAVE complex | en_GB |
dc.subject | Stomata | en_GB |
dc.subject | actin filaments | en_GB |
dc.subject | darkness | en_GB |
dc.subject | Abscisic Acid | en_GB |
dc.subject | Actin Cytoskeleton | en_GB |
dc.subject | Actin-Related Protein 2-3 Complex | en_GB |
dc.subject | Alleles | en_GB |
dc.subject | Arabidopsis | en_GB |
dc.subject | Arabidopsis Proteins | en_GB |
dc.subject | Base Sequence | en_GB |
dc.subject | Bridged Bicyclo Compounds, Heterocyclic | en_GB |
dc.subject | Calcium Chloride | en_GB |
dc.subject | Cytochalasin D | en_GB |
dc.subject | Darkness | en_GB |
dc.subject | Genes, Plant | en_GB |
dc.subject | Models, Biological | en_GB |
dc.subject | Multiprotein Complexes | en_GB |
dc.subject | Mutation | en_GB |
dc.subject | Phenotype | en_GB |
dc.subject | Plant Stomata | en_GB |
dc.subject | Protein Subunits | en_GB |
dc.subject | Thiazolidines | en_GB |
dc.title | Actin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2018-05-14T10:40:22Z | |
dc.identifier.issn | 0028-646X | |
exeter.place-of-publication | England | en_GB |
dc.description | This is the final version of the article. Available from the publisher via the DOI in this record. | en_GB |
dc.identifier.journal | New Phytologist | en_GB |