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dc.contributor.authorIsner, J-C
dc.contributor.authorXu, Z
dc.contributor.authorCosta, JM
dc.contributor.authorMonnet, F
dc.contributor.authorBatstone, T
dc.contributor.authorOu, X
dc.contributor.authorDeeks, MJ
dc.contributor.authorGenty, B
dc.contributor.authorJiang, K
dc.contributor.authorHetherington, AM
dc.date.accessioned2018-05-14T10:40:22Z
dc.date.issued2017-08
dc.description.abstractStomata respond to darkness by closing to prevent excessive water loss during the night. Although the reorganisation of actin filaments during stomatal closure is documented, the underlying mechanisms responsible for dark-induced cytoskeletal arrangement remain largely unknown. We used genetic, physiological and cell biological approaches to show that reorganisation of the actin cytoskeleton is required for dark-induced stomatal closure. The opal5 mutant does not close in response to darkness but exhibits wild-type (WT) behaviour when exposed to abscisic acid (ABA) or CaCl2 . The mutation was mapped to At5g18410, encoding the PIR/SRA1/KLK subunit of the ArabidopsisSCAR/WAVE complex. Stomata of an independent allele of the PIR gene (Atpir-1) showed reduced sensitivity to darkness and F1 progenies of the cross between opal5 and Atpir-1 displayed distorted leaf trichomes, suggesting that the two mutants are allelic. Darkness induced changes in the extent of actin filament bundling in WT. These were abolished in opal5. Disruption of filamentous actin using latrunculin B or cytochalasin D restored wild-type stomatal sensitivity to darkness in opal5. Our findings suggest that the stomatal response to darkness is mediated by reorganisation of guard cell actin filaments, a process that is finely tuned by the conserved SCAR/WAVE-Arp2/3 actin regulatory module.en_GB
dc.description.sponsorshipThis work was supported by grants from the BBSRC (BB/ N001168/1; BB/J002364/1; BBF001177/1), The Gatsby Charitable Foundation and the Leverhulme Trust to A.M.H. and grants from the National Natural Science Foundation of China (nos. 31300213 and 31670408 to K.J.). This work was also supported by the Centre National de la Recherche Scientifique and the Commissariat a l’Energie Atomique et aux Energies Alternatives (for the IR camera) and the European Union Marie Curie FP5 Research Training Network (program no. STRESSIMAGING HNRT-CT-2002-00254 to J.M.C. and B.G.). J.M.C. was in addition supported by a scholarship of the Fundac ~ao para a Ci^encia e Tecnologia, Portugal (grant no. SFRH/BPD/34429/2006).en_GB
dc.identifier.citationVol. 215, pp. 1059 - 1067en_GB
dc.identifier.doi10.1111/nph.14655
dc.identifier.urihttp://hdl.handle.net/10871/32825
dc.language.isoenen_GB
dc.publisherWileyen_GB
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pubmed/28636198en_GB
dc.rights©2017 The Authors. New Phytologist © 2017 New Phytologist Trust This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_GB
dc.subjectArp2/3 complexen_GB
dc.subjectSCAR/WAVE complexen_GB
dc.subjectStomataen_GB
dc.subjectactin filamentsen_GB
dc.subjectdarknessen_GB
dc.subjectAbscisic Aciden_GB
dc.subjectActin Cytoskeletonen_GB
dc.subjectActin-Related Protein 2-3 Complexen_GB
dc.subjectAllelesen_GB
dc.subjectArabidopsisen_GB
dc.subjectArabidopsis Proteinsen_GB
dc.subjectBase Sequenceen_GB
dc.subjectBridged Bicyclo Compounds, Heterocyclicen_GB
dc.subjectCalcium Chlorideen_GB
dc.subjectCytochalasin Den_GB
dc.subjectDarknessen_GB
dc.subjectGenes, Planten_GB
dc.subjectModels, Biologicalen_GB
dc.subjectMultiprotein Complexesen_GB
dc.subjectMutationen_GB
dc.subjectPhenotypeen_GB
dc.subjectPlant Stomataen_GB
dc.subjectProtein Subunitsen_GB
dc.subjectThiazolidinesen_GB
dc.titleActin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness.en_GB
dc.typeArticleen_GB
dc.date.available2018-05-14T10:40:22Z
dc.identifier.issn0028-646X
exeter.place-of-publicationEnglanden_GB
dc.descriptionThis is the final version of the article. Available from the publisher via the DOI in this record.en_GB
dc.identifier.journalNew Phytologisten_GB


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