dc.contributor.author | Bauknecht, P | |
dc.contributor.author | Jékely, G | |
dc.date.accessioned | 2018-05-14T13:35:45Z | |
dc.date.issued | 2015-07-28 | |
dc.description.abstract | Neuropeptides, representing the largest class of neuromodulators, commonly signal by G-protein-coupled receptors (GPCRs). While the neuropeptide repertoire of several metazoans has been characterized, many GPCRs are orphans. Here, we develop a strategy to identify GPCR-peptide pairs using combinatorial screening with complex peptide mixtures. We screened 126 neuropeptides against 87 GPCRs of the annelid Platynereis and identified ligands for 19 receptors. We assigned many GPCRs to known families and identified conserved families of achatin, FMRFamide, RGWamide, FLamide, and elevenin receptors. We also identified a ligand for the Platynereis ortholog of vertebrate thyrotropin-releasing hormone (TRH) receptors, revealing the ancient origin of TRH-receptor signaling. We predicted ligands for several metazoan GPCRs and tested predicted achatin receptors. These receptors were specifically activated by an achatin D-peptide, revealing a conserved mode of activation. Our work establishes an important resource and provides information about the complexity of peptidergic signaling in the urbilaterian. | en_GB |
dc.description.sponsorship | on assays. We also thank
Elizabeth Williams for comments on the manuscript. The research leading to
these results received funding from the European Research Council under
the European Union’s Seventh Framework Programme (FP7/2007-2013)/
European Research Council Grant Agreement 260821. P.B. is supported by
the International Max Planck Research School (IMPRS) ‘‘From Molecules to
Organisms | en_GB |
dc.identifier.citation | Vol. 12, pp. 684 - 693 | en_GB |
dc.identifier.doi | 10.1016/j.celrep.2015.06.052 | |
dc.identifier.uri | http://hdl.handle.net/10871/32841 | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/26190115 | en_GB |
dc.rights | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | en_GB |
dc.subject | Amino Acid Sequence | en_GB |
dc.subject | Animals | en_GB |
dc.subject | Aplysia | en_GB |
dc.subject | Binding Sites | en_GB |
dc.subject | CHO Cells | en_GB |
dc.subject | Cricetinae | en_GB |
dc.subject | Cricetulus | en_GB |
dc.subject | Molecular Docking Simulation | en_GB |
dc.subject | Molecular Sequence Data | en_GB |
dc.subject | Neuropeptides | en_GB |
dc.subject | Polychaeta | en_GB |
dc.subject | Protein Binding | en_GB |
dc.subject | Receptors, G-Protein-Coupled | en_GB |
dc.subject | Receptors, Thyrotropin | en_GB |
dc.title | Large-scale combinatorial deorphanization of Platynereis neuropeptide GPCRs. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2018-05-14T13:35:45Z | |
dc.identifier.issn | 2211-1247, | |
exeter.place-of-publication | United States | en_GB |
dc.description | This is the final version of the article. Available from the publisher via the DOI in this record. | en_GB |
dc.identifier.journal | Cell Reports | en_GB |