A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes.
dc.contributor.author | Shahidi, R | |
dc.contributor.author | Williams, EA | |
dc.contributor.author | Conzelmann, M | |
dc.contributor.author | Asadulina, A | |
dc.contributor.author | Verasztó, C | |
dc.contributor.author | Jasek, S | |
dc.contributor.author | Bezares-Calderón, LA | |
dc.contributor.author | Jékely, G | |
dc.date.accessioned | 2018-05-14T13:42:44Z | |
dc.date.issued | 2015-12-15 | |
dc.description.abstract | Electron microscopy-based connectomics aims to comprehensively map synaptic connections in neural tissue. However, current approaches are limited in their capacity to directly assign molecular identities to neurons. Here, we use serial multiplex immunogold labeling (siGOLD) and serial-section transmission electron microscopy (ssTEM) to identify multiple peptidergic neurons in a connectome. The high immunogenicity of neuropeptides and their broad distribution along axons, allowed us to identify distinct neurons by immunolabeling small subsets of sections within larger series. We demonstrate the scalability of siGOLD by using 11 neuropeptide antibodies on a full-body larval ssTEM dataset of the annelid Platynereis. We also reconstruct a peptidergic circuitry comprising the sensory nuchal organs, found by siGOLD to express pigment-dispersing factor, a circadian neuropeptide. Our approach enables the direct overlaying of chemical neuromodulatory maps onto synaptic connectomic maps in the study of nervous systems. | en_GB |
dc.description.sponsorship | The research leading to these results received funding from the European Research Council under the European Union’s Seventh Framework Programme (FP7/2007-2013)/European Research Council Grant Agreement 260821. This project is supported by the Marie Curie ITN "Neptune", GA 317172, funded under the FP7, PEOPLE Work Programme of the European Commission. | en_GB |
dc.identifier.citation | Vol. 4, article e11147 | en_GB |
dc.identifier.doi | 10.7554/eLife.11147 | |
dc.identifier.uri | http://hdl.handle.net/10871/32843 | |
dc.language.iso | en | en_GB |
dc.publisher | eLife Sciences Publications | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/26670546 | en_GB |
dc.rights | This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. | en_GB |
dc.subject | Platynereis dumerilii | en_GB |
dc.subject | annelid | en_GB |
dc.subject | connectome | en_GB |
dc.subject | immunogold | en_GB |
dc.subject | neuropeptides | en_GB |
dc.subject | neuroscience | en_GB |
dc.subject | Animals | en_GB |
dc.subject | Connectome | en_GB |
dc.subject | Immunohistochemistry | en_GB |
dc.subject | Microscopy, Electron, Transmission | en_GB |
dc.subject | Neurons | en_GB |
dc.subject | Neuropeptides | en_GB |
dc.subject | Polychaeta | en_GB |
dc.subject | Staining and Labeling | en_GB |
dc.title | A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2018-05-14T13:42:44Z | |
dc.identifier.issn | 2050-084X | |
exeter.place-of-publication | England | en_GB |
dc.description | This is the final version of the article. | en_GB |
dc.description | Available from eLife Sciences Publications via the DOI in this record. | en_GB |
dc.identifier.journal | eLife | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ |
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