dc.contributor.author | Evans, CT | |
dc.contributor.author | Payton, O | |
dc.contributor.author | Picco, L | |
dc.contributor.author | Allen, MJ | |
dc.date.accessioned | 2018-11-01T12:58:25Z | |
dc.date.issued | 2018-09-12 | |
dc.description.abstract | Visualization of algal viruses has been paramount to their study and understanding. The direct observation of the morphological dynamics of infection is a highly desired capability and the focus of instrument development across a variety of microscopy technologies. However, the high temporal (ms) and spatial resolution (nm) required, combined with the need to operate in physiologically relevant conditions presents a significant challenge. Here we present a short history of virus structure study and its relation to algal viruses and highlight current work, concentrating on electron microscopy and atomic force microscopy, towards the direct observation of individual algae⁻virus interactions. Finally, we make predictions towards future algal virus study direction with particular focus on the exciting opportunities offered by modern high-speed atomic force microscopy methods and instrumentation. | en_GB |
dc.description.sponsorship | The previously unpublished AFM images shown in this manuscript were generated in the Plymouth Marine Laboratory and University of Exeter Environmental Single Cell Genomics Facility which was supported by the UK’s Natural Environment Research Council (NERC) and The Wolfson Foundation. C.T.E. is supported by an EPSRC and Bristol Nano Dynamics Ltd PhD studentship. | en_GB |
dc.identifier.citation | Vol. 10 (9), article 490 | en_GB |
dc.identifier.doi | 10.3390/v10090490 | |
dc.identifier.uri | http://hdl.handle.net/10871/34590 | |
dc.language.iso | en | en_GB |
dc.publisher | MDPI | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/30213102 | en_GB |
dc.rights | © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | en_GB |
dc.subject | algal virus | en_GB |
dc.subject | atomic force microscopy | en_GB |
dc.subject | electron microscopy | en_GB |
dc.subject | imaging | en_GB |
dc.subject | infection dynamics | en_GB |
dc.subject | virus structure | en_GB |
dc.title | Algal Viruses: The (Atomic) Shape of Things to Come | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2018-11-01T12:58:25Z | |
exeter.place-of-publication | Switzerland | en_GB |
dc.description | This is the final version. Available on open access from MDPI via the DOI in this record | en_GB |
dc.identifier.journal | Viruses | en_GB |