dc.contributor.author | Teague, J | |
dc.contributor.author | Scott, TB | |
dc.contributor.author | Sharma, S | |
dc.contributor.author | Graham, G | |
dc.contributor.author | Allen, MJ | |
dc.date.accessioned | 2018-11-01T13:26:44Z | |
dc.date.issued | 2017-06-02 | |
dc.description.abstract | The development of low-cost, open-source Remotely Operated Vehicle (ROV) systems has provided almost unrestricted access for researchers looking to monitor the marine environment in ever greater resolution. Sampling microbial communities from the marine environment, however, still usually relies on Niskin-bottle sampling (ROV or Conductivity-Temperature-Depth sampler (CTD) based), a method which introduces an inaccuracy and variability that is incompatible with metatranscriptomic analysis, for example. Here, we describe a versatile, easily-replicated platform which achieves in situ mRNA preservation, via the addition of RNAlater to filtered microbial cells, to enhance ROV or CTD functionality. | en_GB |
dc.description.sponsorship | We would like to acknowledge the internally funded PML Research Program (awarded to M.J.A.) and Cabot Institute Innovation Fund (awarded to T.S. and M.J.A.) for providing the funding for this study. | en_GB |
dc.identifier.citation | Vol. 5 (2), article 22 | en_GB |
dc.identifier.doi | 10.3390/jmse5020022 | |
dc.identifier.uri | http://hdl.handle.net/10871/34594 | |
dc.language.iso | en | en_GB |
dc.publisher | MDPI | en_GB |
dc.rights | © 2017 The Author(s). This is an open access article distributed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0). | en_GB |
dc.subject | remotely operated vehicle | en_GB |
dc.subject | metatranscriptomics | en_GB |
dc.subject | Niskin | en_GB |
dc.title | An alternative method to niskin sampling for molecular analysis of the marine environment | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2018-11-01T13:26:44Z | |
dc.description | This is the final version. Available on open access from MDPI via the DOI in this record | en_GB |
dc.identifier.journal | Journal of Marine Science and Engineering | en_GB |