dc.contributor.author | Wagley, S | |
dc.contributor.author | Borne, R | |
dc.contributor.author | Harrison, J | |
dc.contributor.author | Baker-Austin, C | |
dc.contributor.author | Ottaviani, D | |
dc.contributor.author | Leoni, F | |
dc.contributor.author | Vuddhakul, V | |
dc.contributor.author | Titball, RW | |
dc.date.accessioned | 2018-11-12T15:49:06Z | |
dc.date.issued | 2017-09-29 | |
dc.description.abstract | Non-toxigenic V. parahaemolyticus isolates (tdh-/trh-/T3SS2-) have recently been isolated from patients with gastroenteritis. In this study we report that the larvae of the wax moth (Galleria mellonella) are susceptible to infection by toxigenic or non-toxigenic clinical isolates of V. parahaemolyticus. In comparison larvae inoculated with environmental isolates of V. parahaemolyticus did not succumb to disease. Whole genome sequencing of clinical non-toxigenic isolates revealed the presence of a gene encoding a nudix hydrolase, identified as mutT. A V. parahaemolyticus mutT mutant was unable to kill G. mellonella at 24 h post inoculation, indicating a role of this gene in virulence. Our findings show that G. mellonella is a valuable model for investigating screening of possible virulence genes of V. parahaemolyticus and can provide new insights into mechanisms of virulence of atypical non-toxigenic V. parahaemolyticus. These findings will allow improved genetic tests for the identification of pathogenic V. parahaemolyticus to be developed and will have a significant impact for the scientific community. | en_GB |
dc.description.sponsorship | This work was partly supported by Wellcome Trust Institutional Strategic Support Fund (WT097835MF), Wellcome Trust Multi User Equipment Award (WT097835MF) Medical Research Council Clinical Infrastructure Funding (MR/M008924/1) and Biotechnology and Biological Sciences Research Council (BBSRC) funding (BB/N016513/1). | en_GB |
dc.identifier.citation | Vol. 9 (1), pp. 197 - 207 | en_GB |
dc.identifier.doi | 10.1080/21505594.2017.1384895 | |
dc.identifier.uri | http://hdl.handle.net/10871/34736 | |
dc.language.iso | en | en_GB |
dc.publisher | Taylor & Francis | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/28960137 | en_GB |
dc.rights | © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group© Sariqa Wagley, Richard Borne, Jamie Harrison, Craig Baker-Austin, Donatella Ottaviani, Francesca Leoni, Varaporn Vuddhakul, and Richard W. Titball
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | Galleria mellonella | en_GB |
dc.subject | MutT | en_GB |
dc.subject | Vibrio parahaemolyticus | en_GB |
dc.subject | nudix hydrolase | en_GB |
dc.title | Galleria mellonella as an infection model to investigate virulence of Vibrio parahaemolyticus | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2018-11-12T15:49:06Z | |
exeter.place-of-publication | United States | en_GB |
dc.description | This is the final version. Available from Taylor & Francis via the DOI in this record | en_GB |
dc.identifier.journal | Virulence | en_GB |