Splicing factors Sf3A2 and Prp31 have direct roles in mitotic chromosome segregation
dc.contributor.author | Pellacani, C | |
dc.contributor.author | Bucciarelli, E | |
dc.contributor.author | Renda, F | |
dc.contributor.author | Hayward, D | |
dc.contributor.author | Palena, A | |
dc.contributor.author | Chen, J | |
dc.contributor.author | Bonaccorsi, S | |
dc.contributor.author | Gatti | |
dc.contributor.author | Wakefield, J | |
dc.contributor.author | Gatti, M | |
dc.contributor.author | Somma, MP | |
dc.date.accessioned | 2019-01-22T16:03:27Z | |
dc.date.issued | 2018-11-26 | |
dc.description.abstract | Several studies have shown that RNAi-mediated depletion of splicing factors (SFs) results in mitotic abnormalities. However, it is currently unclear whether these abnormalities reflect defective splicing of specific pre-mRNAs or a direct role of the SFs in mitosis. Here, we show that two highly conserved SFs, Sf3A2 and Prp31, are required for chromosome segregation in both Drosophila and human cells. Injections of anti-Sf3A2 and anti-Prp31 antibodies into Drosophila embryos disrupt mitotic division within 1 min, arguing strongly against a splicing-related mitotic function of these factors. We demonstrate that both SFs bind spindle microtubules (MTs) and the Ndc80 complex, which in Sf3A2- and Prp31-depleted cells is not tightly associated with the kinetochores; in HeLa cells the Ndc80/HEC1-SF interaction is restricted to the M phase. These results indicate that Sf3A2 and Prp31 directly regulate interactions among kinetochores, spindle microtubules and the Ndc80 complex in both Drosophila and human cells. | en_GB |
dc.description.sponsorship | Italian Association for Cancer Research | en_GB |
dc.description.sponsorship | Italian Association for Cancer Research | en_GB |
dc.identifier.citation | Vol. 7, article e40325 | en_GB |
dc.identifier.doi | 10.7554/eLife.40325 | |
dc.identifier.grantnumber | IG16020 | en_GB |
dc.identifier.grantnumber | 20528 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/35561 | |
dc.language.iso | en | en_GB |
dc.publisher | eLife Sciences Publications | en_GB |
dc.rights | This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. | en_GB |
dc.title | Splicing factors Sf3A2 and Prp31 have direct roles in mitotic chromosome segregation | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-01-22T16:03:27Z | |
dc.identifier.issn | 2050-084X | |
dc.description | This is the final version. Available from eLife Sciences Publications via the DOI in this record. | en_GB |
dc.identifier.journal | eLife | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2018-11-14 | |
rioxxterms.funder | Biotechnology and Biological Sciences Research Council | en_GB |
rioxxterms.funder | Biotechnology and Biological Sciences Research Council | en_GB |
rioxxterms.identifier.project | BB/K017837/1 | en_GB |
rioxxterms.identifier.project | PhD studentship | en_GB |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2018-11-14 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-01-22T15:47:51Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2019-01-22T16:03:30Z | |
refterms.panel | A | en_GB |
refterms.depositException | publishedGoldOA | |
rioxxterms.funder.project | 3e784547-a230-4688-ac26-2a9a474a4c3e | en_GB |
rioxxterms.funder.project | e44418f7-527b-4475-a115-6e941aee3ab5 | en_GB |
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